Attachment of biomolecules (protein and DNA) to amino-functionalized carbon nanotubes
Attachment of biomolecules (protein and DNA) to amino-functionalized carbon nanotubes
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DOI：
10.1016/s1872-5805(08)60053-0
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发表时间：
2009-12-01
影响因子：
5.7
通讯作者：
Srivastava, O. N.
中科院分区：
文献类型：
--
作者：
Awasthi, Kalpana;Singh, D. P.;Srivastava, O. N.
一种高效的生物分子附着方法[j]。g.牛血清白蛋白（BSA）蛋白和脱氧核糖核酸（DNA）]转化为氨基功能化的多壁碳纳米管（f-MWCNTs）。将苯-二茂铁溶液在850℃左右的氩气气氛中喷雾热解制备MWCNTs，然后在纳米管表面引入羧基进行化学修饰，使其与氨基官能化。这个过程包括乙二胺与羧基的直接偶联，通过酰胺的形成引入氨基。通过扫描电镜、透射电镜和傅里叶变换红外光谱对合成的含BSA蛋白和DNA的MWCNTs、f-MWCNTs和氨基f-MWCNTs进行了表征，证实了生物分子（BSA蛋白和DNA）附着在氨基f-MWCNTs上。
An efficient method for the attachment of biomolecules [e. g. bovine serum albumin (BSA) protein and deoxyribonucleic acid (DNA)] to amino-group-functionalized multiwalled carbon nanotubes (f-MWCNTs) was reported. MWCNTs were prepared by spray pyrolysis of a benzene-ferrocene solution in argon atmosphere at similar to 850 degrees C followed by functionalization with an amino group by chemical modification of carboxylic groups introduced on the nanotube surface. This process involves a direct coupling of ethylenediamine with carboxylic groups to introduce amino groups by amide formation. The as-synthesized MWCNTs, f-MWCNTs, and amino f-MWCNTs with BSA protein and DNA were characterized by scanning and transmission electron microscopy, and Fourier transform infrared spectroscopy, which confirm the attachment of biomolecules (BSA protein and DNA) to the amino f-MWCNTs.