An oxygen-releasing device to improve the survival of mesenchymal stem cells in tissue engineering
An oxygen-releasing device to improve the survival of mesenchymal stem cells in tissue engineering
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DOI：
10.1088/1758-5090/ab332a
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发表时间：
2019-10-01
期刊：
影响因子：
9
通讯作者：
Kang, Y. James
中科院分区：
文献类型：
--
作者：
Lu, Zuyan;Jiang, Xia;Kang, Y. James
向细胞构建体的内部区域提供氧气以支持细胞增殖和代谢是涉及干细胞的组织工程的主要挑战。因此，开发结合氧气释放材料以增加局部氧气供应的设备是解决此限制的关键。本文中，我们设计并开发了一种3D打印的氧气释放装置，该装置由藻酸盐水凝胶支架组成，并结合了生成氧的生物材料（过氧化钙），以改善微环境的氧气供应，用于培养脂肪糖组织培养的脂肪组织。结果表明，3D打印的氧气释放装置减轻了缺氧，维持氧气的可用性并确保嵌入细胞的扩散，同时还减少了缺氧诱导的细胞凋亡。引入这种3D打印的氧气释放装置可以增强嵌入式干细胞的存活。
Supplying oxygen to inner areas of cell constructs to support cell proliferation and metabolism is a major challenge in tissue engineering involving stem cells. Developing devices that incorporate oxygen release materials to increase the availability of the localized oxygen supply is therefore key to addressing this limitation. Herein, we designed and developed a 3D-printed oxygen-releasing device composed of an alginate hydrogel scaffold combined with an oxygen-generating biomaterial (calcium peroxide) to improve the oxygen supply of the microenvironment for culturing adipose tissue-derived stem cells. The results demonstrated that the 3D-printed oxygen-releasing device alleviated hypoxia, maintained oxygen availability, and ensured proliferation of the embedded cells, whilst also reducing hypoxia-induced apoptosis. The introduction of this 3D-printed oxygen-releasing device could enhance the survival of embedded stem cells.