SENSITIVITY OF OSTEOCYTES TO BIOMECHANICAL STRESS IN-VITRO
SENSITIVITY OF OSTEOCYTES TO BIOMECHANICAL STRESS IN-VITRO
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DOI：
10.1096/fasebj.9.5.7896017
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发表时间：
1995-03-01
期刊：
影响因子：
4.8
通讯作者：
BURGER, EH
中科院分区：
文献类型：
--
作者：
KLEINNULEND, J;VANDERPLAS, A;BURGER, EH
一个多世纪以来，人们已经知道骨组织通过结构和质量的变化来适应功能应激，然而，应激如何转化为骨形成和吸收的细胞活动的机制尚不清楚。我们研究了从胚胎鸡颅骨分离的骨细胞对间歇性静水压缩和脉动流体的反应，并比较了它们对成骨细胞和骨膜成纤维细胞的反应。骨细胞，而不是成骨细胞或骨膜成纤维细胞，对1小时的脉动液体流动产生反应，并持续释放前列腺素E(2)。间歇静水压缩在较小程度上刺激前列腺素的产生：在骨细胞中，6和24小时后，在成骨细胞中，6小时后。这些数据证明骨细胞是骨中参与机械应力转化为生物反应的最机械敏感细胞。研究结果支持了骨应力引起腔隙-小管系统液体流动的假设，这刺激骨细胞产生调节骨代谢的因子。
It has been known for more than a century that bone tissue adapts to functional stress by changes in structure and mass, However, the mechanism by which stress is translated into cellular activities of bone formation and resorption is unknown, We studied the response of isolated osteocytes derived from embryonic chicken calvariae to intermittent hydrostatic compression as well as pulsating fluid flow, and compared their response to osteoblasts and periosteal fibroblasts. Osteocytes, but not osteoblasts or periosteal fibroblasts, reacted to 1 h pulsating fluid flow with a sustained release of prostaglandin E(2). Intermittent hydrostatic compression stimulated prostaglandin production to a lesser extent: after 6 and 24 h in osteocytes and after 6 h in osteoblasts. These data provide evidence that osteocytes are the most mechanosensitive cells in bone involved in the transduction of mechanical stress into a biological response. The results support the hypothesis that stress on bone causes fluid flow in the lacunar-canalicular system, which stimulates the osteocytes to produce factors that regulate bone metabolism.