Construction and characterization of Escherichia coli disruptants defective in the yaeM gene
Construction and characterization of Escherichia coli disruptants defective in the yaeM gene
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DOI：
10.1271/bbb.63.776
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发表时间：
1999-04-01
影响因子：
1.6
通讯作者：
Seto, H
中科院分区：
文献类型：
--
作者：
Kuzuyama, T;Takahashi, S;Seto, H
构建了 yaeM 基因缺陷的大肠杆菌破坏株，该基因位于染色体图上的 4.2 分钟处。干扰物显示出 2-C-甲基赤藓糖醇营养缺陷型，2-C-甲基-D-赤藓糖醇 4-磷酸的游离醇是非甲羟戊酸途径中的生物合成前体。这一结果清楚地表明，在大肠杆菌中，yaeM基因确实参与了该途径。
Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli.