Replication of porcine circovirus type 1 requires two proteins encoded by the viral rep gene
Replication of porcine circovirus type 1 requires two proteins encoded by the viral rep gene
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DOI：
10.1006/viro.2000.0730
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发表时间：
2001-01-20
期刊：
影响因子：
3.7
通讯作者：
Hillenbrand, B
中科院分区：
文献类型：
--
作者：
Mankertz, A;Hillenbrand, B
猪圆环病毒1型（PCV 1）的rep基因是病毒DNA复制所必需的。截断或引入点突变到四个保守的序列基序导致的Rep作为复制启动子的失活。rep的转录起始于核苷酸767 +/-10 bp。内含子（核苷酸1176至1558）通过剪接去除。这导致截短蛋白的合成，其被称为Rep'（19.2kDa）。由于移码，Rep'的最后48个氨基酸偏离35.6-kDa全长Rep蛋白的C-末端。在PCV 1感染的细胞以及用携带rep基因的质粒转染的细胞中，通过实时PCR证明了全长和剪接的rep转录物的存在。与通过滚环复制的其他病毒相反，Rep蛋白单独不能促进复制：Rep和Rep'一起构成PCV 1的功能性复制起始因子。(C)北京：科学出版社.
The rep gene of porcine circovirus type 1 (PCV1) is indispensable for replication of viral DNA. Truncation or introduction of point mutations into four conserved sequence motifs led to inactivation of Rep as replication initiator. Transcription of rep starts at nucleotide 767 +/- 10 bp. An intron (nucleotides 1176 to 1558) is removed by splicing. This leads to synthesis of a truncated protein, which was termed Rep' (19.2 kDa). Because of a frameshift, the last 48 amino acids of Rep' deviate from the C-terminus of the 35.6-kDa full-length Rep protein. The presence of full-length and spliced rep transcripts was demonstrated in PCV1-infected cells as well as in cells transfected with a plasmid carrying the rep gene by real-time PCR. In contrast to other viruses replicating via a rolling circle, Rep protein alone cannot promote replication: Rep and Rep' together comprise the functional replication initiator factor of PCV1. (C) 2001 Academic Press.