Hemolytic phospholipase Rv0183 of Mycobacterium tuberculosis induces inflammatory response and apoptosis in alveolar macrophage RAW264.7 cells.
Hemolytic phospholipase Rv0183 of Mycobacterium tuberculosis induces inflammatory response and apoptosis in alveolar macrophage RAW264.7 cells.
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DOI：
10.1139/w10-079
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发表时间：
2010-10
影响因子：
2.8
通讯作者：
Guangxian Xu;H. Jia;Yong Li;Xiaoming Liu;Min Li;Yujiong Wang
中科院分区：
文献类型：
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作者：
Guangxian Xu;H. Jia;Yong Li;Xiaoming Liu;Min Li;Yujiong Wang
磷脂代谢途径是结核分枝杆菌这种典型的胞内细菌最重要的生理途径之一。溶血性磷脂酶唇基因（Rv0183）是已证实在结核分枝杆菌的磷脂代谢中起关键作用的24个磷脂酶基因之一。采用定量RT-PCR和流式细胞术研究Rv0183基因在小鼠肺泡巨噬细胞RAW264.7细胞中持续表达后对炎症反应的免疫学和病原学意义。我们的研究结果表明，Rv0183的时间依赖性异位表达显著提高了这些肺泡巨噬细胞中IL-6、NF-κB、TLR-2、TLR-6、TNFα和MyD88的表达。此外，Rv0183在体外持续表达诱导RAW264.7细胞凋亡。这些结果表明，Rv0183的表达可诱导宿主细胞的炎症反应和细胞凋亡，提示Rv0183可能在结核分枝杆菌感染的毒力和发病机制中发挥重要作用。
The metabolic pathway of phospholipids is one of the most important physiologic pathways in Mycobacterium tuberculosis, a typical intracellular bacterium. The hemolytic phospholipase lip gene (Rv0183) is one of 24 phospholipase genes that have been demonstrated to play critical roles in the metabolism of phospholipids in M. tuberculosis. Quantitative RT-PCR and flow cytometry were used to elucidate the immunological and pathogenic implications of the Rv0183 gene on the inflammatory response following persistent expression of Rv0183 in mouse alveolar macrophage RAW264.7 cells. Our results demonstrate that a time-course-dependent ectopic expression of Rv0183 significantly elevated the expression of IL-6, NF-κB, TLR-2, TLR-6, TNFα, and MyD88 in these alveolar macrophage cells. Furthermore, the persistent expression of Rv0183 induced RAW264.7 cell apoptosis in vitro. These findings demonstrate that the expression of Rv0183 induces an inflammatory response and cell apoptosis in the host cells, suggesting that Rv0183 may play an important role in the virulence and pathogenesis of M. tuberculosis infection.