Liposome loaded phage cocktail: Enhanced therapeutic potential in resolving Klebsiella pneumoniae mediated burn wound infections
Liposome loaded phage cocktail: Enhanced therapeutic potential in resolving Klebsiella pneumoniae mediated burn wound infections
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DOI：
10.1016/j.burns.2017.03.029
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发表时间：
2017-11-01
期刊：
影响因子：
2.7
通讯作者：
Chhibber, Sanjay
中科院分区：
文献类型：
--
作者：
Chadha, Parul;Katare, Om Prakash;Chhibber, Sanjay
背景：肺炎克雷伯菌是烧伤创面感染的主要病原菌之一，多重耐药菌株的流行使情况更加复杂。近年来，越来越多的人对噬菌体疗法作为一种对抗感染的手段感兴趣。为了克服噬菌体疗法的缺点，本研究评价了脂质体作为噬菌体载体治疗烧伤创面感染的可能性。方法：建立BALB/c小鼠烧伤创面肺炎克雷伯菌感染模型。比较了游离噬菌体鸡尾酒与脂质体包裹噬菌体鸡尾酒在解决小鼠烧伤创面感染过程中的疗效。结果：脂质体包裹噬菌体鸡尾酒对小鼠血液和主要器官的细菌负荷有较高的降低作用。与非脂质体游离噬菌体鸡尾酒相比，这伴随着整个感染过程的更快解决。脂质体延长了噬菌体在体内的滞留时间，从而增强了疗效。结论：脂质体包裹噬菌体鸡尾酒对肺炎克雷伯菌介导性感染具有一定的治疗潜力。因此，这一策略可以作为一种有效的方法来治疗克雷伯氏菌介导的烧伤创面感染，这些患者对常规抗生素治疗无效。(C)2017年爱思唯尔有限公司和ISBI。版权所有。
Background: Klebsiella pneumoniae is one of the predominant pathogens in burn wound infections, and prevalence of multidrug resistant strains has further complicated the situation. An increased interest in phage therapy as a means of combating infection has been accruing in recent years. In order to overcome the drawbacks associated with phage therapy, the present study was conducted to evaluate the potential of liposomes as a delivery vehicle for phage in the treatment of burn wound infection.Methods: Burn wound infection with Klebsiella pneumoniae B5055 was established in BALB/c mice. The therapeutic efficacy of free phage cocktail in comparison to liposome entrapped phage cocktail in resolving the course of burn wound infection in mice was evaluated.Results: The results depicted that mice treated with liposomal entrapped phage cocktail showed higher reduction in bacterial load in blood and major organs. This was accompanied with faster resolution of the entire infection process as compared to non-liposomal free phage cocktail. The liposomes increased phage retention time in vivo thus potentiating efficacy. Liposomal phage preparation was able to protect all the test animals from death even when there was a delay of 24h in instituting the therapy.Conclusion: The results showed the potential of liposome entrapped phage cocktail for treating Klebsiella pneumoniae mediated infections. Thus, this strategy can serve as an effective approach for treating Klebsiella mediated burn wound infections in individuals who do not respond to conventional antibiotic therapy. (C) 2017 Elsevier Ltd and ISBI. All rights reserved.