Labeling Human Mesenchymal Stem Cells with Fluorescent Contrast Agents: the Biological Impact
Labeling Human Mesenchymal Stem Cells with Fluorescent Contrast Agents: the Biological Impact
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DOI：
10.1007/s11307-010-0322-0
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发表时间：
2011-02-01
影响因子：
3.1
通讯作者：
Daldrup-Link, Heike E.
中科院分区：
文献类型：
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作者：
Boddington, Sophie E.;Sutton, Elizabeth J.;Daldrup-Link, Heike E.
本研究旨在探讨荧光染料DiD和纳米氧化铁标记的人骨髓间充质干细胞（human mesenchymal stem cell，hMSC）对软骨形成的影响。hMSCs进行共聚焦显微镜，光学成像（OI），和磁共振（MR）成像。软骨形成由转化生长因子-b诱导，并通过组织病理学和糖胺聚糖（GAG）的产生来证实。标记和未标记hMSCs的数据用t检验比较，共聚焦显微镜证实细胞摄取DiD和阿魏酸。DiD标记在OI上引起显著的荧光，并且ferucarbotran标记在MR图像上引起显著的T2* 效应。与未标记的对照相比，标记的MSC的后代在软骨形成分化后表现出相似的软骨细胞形态，但标记的细胞表现出显著减少的GAG产生（p < 0.05）diD和DiD/ferucarbotran标记的hMSC不干扰细胞活力或形态分化成软骨细胞，但与未标记的细胞相比，标记的细胞表现出显著减少的GAG产生。
This study aims to determine the effect of human mesenchymal stem cell (hMSC) labeling with the fluorescent dye DiD and the iron oxide nanoparticle ferucarbotran on chondrogenesis.hMSCs were labeled with DiD alone or with DiD and ferucarbotran (DiD/ferucarbotran). hMSCs underwent confocal microscopy, optical imaging (OI), and magnetic resonance (MR) imaging. Chondrogenesis was induced by transforming growth factor-b and confirmed by histopathology and glycosaminoglycan (GAG) production. Data of labeled and unlabeled hMSCs were compared with a t test.Cellular uptake of DiD and ferucarbotran was confirmed with confocal microscopy. DiD labeling caused a significant fluorescence on OI, and ferucarbotran labeling caused a significant T2* effect on MR images. Compared to nonlabeled controls, progenies of labeled MSCs exhibited similar chondrocyte morphology after chondrogenic differentiation, but the labeled cells demonstrated significantly reduced GAG production (p < 0.05).DiD and DiD/ferucarbotran labeling of hMSC does not interfere with cell viability or morphologic differentiation into chondrocytes, but labeled cells exhibit significantly less GAG production compared to unlabeled cells.