Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac
Sensitive HPLC–fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac
复制标题
高效液相色谱-荧光法检测大鼠脑和血液微透析液中 DIB-Cl 标记的吗啡及其在吗啡与双氯芬酸药代动力学相互作用的初步研究中的应用
DOI：
10.1007/s00216-008-2070-4
复制
发表时间：
2008
影响因子：
4.3
通讯作者：
K. Nakashima
中科院分区：
文献类型：
--
作者：
M. Wada;Chiaki Yokota;Y. Ogata;N. Kuroda;H. Yamada;K. Nakashima
以4-（4，5-二苯基-1H-咪唑-2-基）苯甲酰氯（DIB-Cl）为标记物，建立了测定大鼠脑组织和血液微透析液中吗啡（莫尔）的高效液相色谱-荧光分析法。在温和的反应条件下（在室温下10分钟）用DIB-Cl标记莫尔。在十八烷基硅胶（ODS）柱上，使用CH 3CN/0.1 M醋酸盐缓冲液（pH 5.4）在14分钟内进行DIB-Mor的分离。在信噪比为3时，脑和血液微透析液中莫尔的检测限分别为0.4和0.6 ng mL−1。该方法已成功应用于莫尔与双氯芬酸潜在药动学相互作用的初步研究。
A sensitive high-performance liquid chromatography (HPLC)–fluorescence method for determination of morphine (Mor) in rat brain and blood microdialysates was developed using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a label. Mor was labeled with DIB-Cl under mild reaction conditions (at room temperature for 10 min). The separation of DIB-Mor was carried out on an octadecylsilica (ODS) column with CH3CN/0.1 M acetate buffer (pH 5.4) within 14 min. The detection limits of Mor in brain and blood microdialysates at a signal-to-noise ratio of 3 were 0.4 and 0.6 ng mL−1, respectively. The proposed method was successfully applied to the preliminarily study of potential pharmacokinetic interaction between Mor and diclofenac.