Direct production of commodity chemicals from lignocellulose using Myceliophthora thermophila
Direct production of commodity chemicals from lignocellulose using Myceliophthora thermophila
复制标题
使用嗜热毁丝霉直接从木质纤维素生产商品化学品
DOI：
10.1016/j.ymben.2019.05.007
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发表时间：
2020-09-01
影响因子：
8.4
通讯作者：
Tian, Chaoguang
中科院分区：
文献类型：
--
作者：
Li, Jingen;Lin, Liangcai;Tian, Chaoguang
从可再生植物生物质生产燃料和化学品已被提议作为全球可持续发展的可行战略。然而，生物炼制的经济效率较低。在这里，通过代谢工程，Myceliophthora thermophila，一种纤维素分解嗜热真菌，被构建成一个平台，可以有效地将木质纤维素转化为重要的大宗化学品-四碳1，4-二酸（苹果酸和琥珀酸），生物聚合物的构建块-而不需要额外的水解酶。在补料分批发酵期间，来自结晶纤维素的滴度> 200 g/L，来自植物生物质（玉米芯）的滴度为110 g/L。我们的研究代表了综合生物加工技术的一个里程碑，并为生物质化学品和燃料的成本效益生产提供了一个新的和有前途的系统。
The production of fuels and chemicals from renewable plant biomass has been proposed as a feasible strategy for global sustainable development. However, the economic efficiency of biorefineries is low. Here, through metabolic engineering, Myceliophthora thermophila, a cellulolytic thermophilic fungus, was constructed into a platform that can efficiently convert lignocellulose into important bulk chemicals-four carbon 1, 4-diacids (malic and succinic acid), building blocks for biopolymers-without the need for extra hydrolytic enzymes. Titers of > 200 g/L from crystalline cellulose and 110 g/L from plant biomass (corncob) were achieved during fed-batch fermentation. Our study represents a milestone in consolidated bioprocessing technology and offers a new and promising system for the cost-effective production of chemicals and fuels from biomass.