Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy
Magnetically Triggered Release From Giant Unilamellar Vesicles: Visualization By Means Of Confocal Microscopy
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DOI：
10.1021/jz2000936
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发表时间：
2011-04-07
影响因子：
5.7
通讯作者：
Baglioni, Piero
中科院分区：
文献类型：
--
作者：
Nappini, Silvia;Al Kayal, Tamer;Baglioni, Piero
通过共焦激光扫描显微镜 (CLSM) 在低频交变磁场 (LF-AMP) 下研究了负载 Alexa 荧光染料的磁性巨型单层囊泡 (GUV) 的磁触发释放。制备了包覆罗丹明B异硫氰酸酯的核/壳钴铁氧体纳米颗粒(MP@SiO2(RITC))并将其吸附在GUV膜上。通过3D-CLSM投影确定MP@SiO2-(RITC)在巨型脂质囊泡上的位置和分布，并通过CLSM时间分辨实验研究它们对LF-AMF下的释放特性和GUV渗透性的影响。我们表明，在 LF-AMF 暴露过程中荧光染料的释放机制是由磁性纳米粒子能量和机械振动引起的，这促进了 GUV 膜的扰动而不使其崩溃。
Magnetically triggered release from magnetic giant unilamellar vesicles (GUVs) loaded with Alexa fluorescent dye was studied by means of confocal laser scanning microscopy (CLSM) under a low-frequency alternating magnetic field (LF-AMP). Core/shell cobalt ferrite nanoparticles coated with rhodamine B isothiocyanate (MP@SiO2(RITC)) were prepared and adsorbed on the GUV membrane. The MP@SiO2-(RITC) location and distribution on giant lipid vesicles were determined by 3D-CLSM projections, and their effect on the release properties and GUV permeability under a LF-AMF was investigated by CLSM time-resolved experiments. We show that the mechanism of release of the fluorescent dye during the LF-AMF exposure is induced by magnetic nanoparticle energy and mechanical vibration, which promote the perturbation of the GUV membrane without its collapse.