Isolate-specific and broad-spectrum QTLs are involved in the control of clubroot in Brassica oleracea
Isolate-specific and broad-spectrum QTLs are involved in the control of clubroot in Brassica oleracea
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DOI：
10.1007/s00122-003-1580-x
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发表时间：
2004-05-01
影响因子：
5.4
通讯作者：
Manzanares-Dauleux, MJ
中科院分区：
文献类型：
--
作者：
Rocherieux, J;Glory, P;Manzanares-Dauleux, MJ
甘蓝根茎病是由芸苔Plasmodiophora brassicae引起的一种危害较大的蔬菜病害。利用C10（抗性羽衣甘蓝）和hdem（易感西兰花）杂交组合F-1和F-2/3的后代，研究了5个油菜单抗的遗传控制和基因座定位。利用RFLP、随机标记和特异pcr标记构建遗传图谱。199个位点被组装成9个连锁组，覆盖1226.3 cM。采用4株单孢子分离株和1株田间分离株，在控制条件下对F-3家族进行抗性鉴定。共有9个基因组区域检测到棍棒病抗性。根据分离物的不同，鉴定出2 ~ 5个qtl。根据分离物的不同，qtl占总表型变异的比例在70% ~ 88%之间。其中一个qtl （Pb-Bo1）在所有分离株中均检测到，解释了20.7-80.7%的表型变异。Pb-Bo1对3株菌株有主要影响，但对后2株的影响较弱。仅在1株分离物中鉴定出5个影响较小的qtl。为了构建抗棍棒病品种，在选择基因组区域用于标记辅助选择策略时，应同时考虑广谱和分离特异性qtl的存在。
Clubroot, caused by Plasmodiophora brassicae, is one of the most damaging diseases of vegetable Brassica crops in the world. In this study, genetic control and mapping of loci implied in quantitative resistance against five isolates of P. brassicae were studied in the F-1 and F-2/3 progenies of the cross C10 (resistant kale)xHDEM (susceptible broccoli). A genetic map was constructed using RFLP, random and specific PCR-based markers. The 199 loci were assembled into nine linkage groups covering 1,226.3 cM. The F-3 families were assessed for resistance under controlled conditions with four single-spore isolates and one field isolate. A total of nine genomic regions were detected for clubroot resistance. Depending on the isolate, two to five QTLs were identified. The total phenotypic variation accounted for by QTLs ranged from 70% to 88% depending on the isolate. One of the QTLs (Pb-Bo1) was detected in all isolates and explained 20.7-80.7% of the phenotypic variation. Pb-Bo1 had a major effect on three isolates but this effect was weaker for the last two. Five QTLs with minor effect were identified in only one isolate. To construct clubroot resistant varieties, the existence of both broad-spectrum and isolate-specific QTLs should be taken into account for the choice of genomic regions to use in a marker-assisted selection strategy.