Preclinical study of near-infrared-guided sentinel lymph node mapping of the porcine lung.
Preclinical study of near-infrared-guided sentinel lymph node mapping of the porcine lung.
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DOI：
10.1016/j.athoracsur.2012.08.101
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发表时间：
2013-01
影响因子：
4.6
通讯作者：
Colson, Yolonda L.
中科院分区：
文献类型：
--
作者：
Khullar, Onkar V.;Gilmore, Denis M.;Matsui, Aya;Ashitate, Yoshitomo;Colson, Yolonda L.
The presence of lymph node metastasis is the most important prognostic factor in early non-small cell lung cancer. Our objective was to develop a rapid, simple, and reliable method for thoracic sentinel lymph node (SLN) identification using near-infrared fluorescence imaging and clinically available contrast agents. Indocyanine green (ICG) was reconstituted in saline, human serum albumin, human fresh frozen plasma, and autologous porcine plasma was evaluated for optimal formulation and dosing for SLN within porcine lungs. Animals were imaged using the FLARE™ imaging system. SLN identification rate, time to identification and fluorescence intensity of the SLN, bronchus, and background were measured. SLN identification rates varied widely ranging from 33% to 100% as a function of the carrier used for ICG reconstitution. No significant difference was noted in SLN fluorescence intensity, however bronchial intensity was significantly higher with ICG:albumin, which resulted in the lowest rate of SLN identification. Subsequent evaluation with 125μM and 250μM ICG:porcine plasma resulted in identification of strongly fluorescent SLNs with identification rates of 93% and 100% and median signal-to-background ratios of 8.5 and 12.15, respectively, in <2 minutes in situ. Near-infrared fluorescence imaging with ICG is a reliable method for SLN mapping in the lung with high sensitivity. Mixing of ICG with plasma resulted in strong SLN fluorescence signal with reliable identification rates.
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影响因子：
2.5
作者：
Schaafsma, Boudewijn E.;Mieog, J. Sven D.;Hutteman, Merlijn;Van der Vorst, Joost R.;Kuppen, Peter J. K.;Lowik, Clemens W. G. M.;Frangioni, John V.;Van de Velde, Cornelis J. H.;Vahrmeijer, Alexander L.
通讯作者：
Vahrmeijer, Alexander L.
DOI：
10.1007/s00464-006-9062-2
发表时间：
2007-07-01
影响因子：
3.1
作者：
Ishikawa, K.;Yasuda, K.;Kitano, S.
通讯作者：
Kitano, S.
影响因子：
3.7
作者：
Yamashita, Shin-ichi;Tokuishi, Keita;Kawahara, Katsunobu
通讯作者：
Kawahara, Katsunobu
影响因子：
9
作者：
Morton, DL;Cochran, AJ;Wang, HJ
通讯作者：
Wang, HJ
影响因子：
3.7
作者：
Tanaka, Eiichi;Choi, Hak Soo;Frangioni, John V.
通讯作者：
Frangioni, John V.