Metastatic inefficiency in mice bearing B16 melanomas.
Metastatic inefficiency in mice bearing B16 melanomas.
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DOI：
10.1038/bjc.1982.6
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发表时间：
1982-01
影响因子：
8.8
通讯作者：
Holmes, J C
中科院分区：
文献类型：
--
作者：
Weiss, L;Mayhew, E;Rapp, D G;Holmes, J C
当静脉注射时。在小鼠体内，B16黑色素瘤细胞的F10亚系在3周内产生的肺癌明显多于F101.r-6亚系的细胞。然而，在携带这些亚系产生的肌肉内肿瘤的动物中，F10细胞的高肺定植潜力并未实现，在未经治疗的原发癌动物中，没有观察到自然肺转移形成的显著差异，即使它们进展到垂死状态。推拿IM。来自这两个亚系的肿瘤在转移方面没有变化，血液中可通过生物检测检测到的癌细胞数量也没有变化。相反，按摩增加了来自侵袭性BL6亚系和B16野生型细胞的肿瘤的转移，在野生型的情况下，增加了循环癌细胞的数量。体外实验表明，来自非肿瘤动物的血细胞对这两种亚系都有毒性，但对F10的毒性小于对F101.r-6的毒性。此外，在静脉注射后。注射放射性标记细胞后，受体肺中保留的F10亚系多于F101。r-6。尽管F10亚系在血管内注射后具有这些明显的转移优势，但来自IM的自然转移的发生率。F10和F101.r-6肿瘤相似，表明F10比F101.r-6细胞进入循环的细胞少得多。因此，F10细胞较高的定植能力与其血管内转移能力不匹配，因为转移效率是由转移过程中最低效率的步骤决定的。
When injected i.v. into mice, the F10 subline of B16 melanoma cells produced significantly more lung tumours over a 3-week period than cells of the F101.r-6 subline. However, in animals bearing intramuscular tumours produced by these sublines, the high pulmonary-colonization potential of the F10 cells was not realized, and no significant differences in natural pulmonary metastasis formation were observed in animals with untreated primary cancers, even when they progressed to the moribund state. Massage of i.m. tumours derived from the two sublines produced no change in metastasis and no changes in the numbers of cancer cells in the blood detectable by bioassay. In contrast, massage increased metastasis from tumours derived from an invasive BL6 subline and B16 wild-type cells and, in the case of the wild-type, the numbers of circulating cancer cells. In vitro experiments show that blood cells from non-tumour-bearing animals are toxic to both sublines; but less to F10 than to F101.r-6. In addition, after i.v. injection of radiolabelled cells, more of the F10 subline were retained in the lungs of recipients than the F101.r-6. In spite of these apparent metastatic advantages of the F10 subline following intravasation, the incidence of natural metastases from i.m. F10 and F101.r-6 tumours was similar, suggesting that substantially fewer F10 than F101.r-6 cells gained access to the circulation. Thus, the higher colonization potential of the F10 cells was not matched by its intravasation potential, since metastatic efficiency is determined by the least efficient step in the metastatic process.