Cytochemical calcium distribution in secretory ameloblasts of the rat in relation to enamel mineralization.
Cytochemical calcium distribution in secretory ameloblasts of the rat in relation to enamel mineralization.
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与牙釉质矿化相关的大鼠分泌成釉细胞中的细胞化学钙分布。
DOI：
10.1159/000146183
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Ashrafi,SH
中科院分区：
文献类型：
--
作者：
Chen,S;Eisenmann,DR;Zaki,AE;Ashrafi,SH
本文研究了氟或钴短暂干扰大鼠前牙釉质矿化的成釉细胞内钙的分布。对细胞膜、线粒体和分泌颗粒中的焦锑酸钙沉淀物进行了形态计量学分析。矿化干扰的特征是在注射后1h内，未矿化的釉质基质在Tomes‘s突的分泌区堆积。氟诱导的矿化障碍并不伴随钙浓度和分布的明显变化。氟化物可能会改变有机基质的合成和分泌，从而影响其矿化能力。用钴处理的分泌性成釉细胞显示出广泛的钙干扰基础，特别是与细胞膜和分泌颗粒相关的钙干扰。分泌成釉细胞可能通过涉及细胞膜和分泌颗粒的机制来主动控制钙对牙釉质的有效性。
Calcium distribution in secretory ameloblasts was studied in rat incisor enamel in which mineralization was temporarily disturbed by injection of either fluoride or cobalt. Pyroantimonate precipitates of calcium were analysed morphometrically in regions of the cell membranes, mitochondria and secretory granules. The disturbances in mineralization were characterized by accumulations of unmineralized enamel matrix at the secretory regions of Tomes’ process within 1 h after injection. Fluoride-induced disturbances in mineralization were not accompanied by marked changes in calcium concentration and distribution. It may be that fluoride causes alterations in the synthesis and secretion of the organic matrix which affects its ability to mineralize. Secretory ameloblasts treated with cobalt showed a broad basis for interference with calcium, in particular that which is associated with cell membranes and secretory granules. Secretory ameloblasts may be actively controlling the availability of calcium to enamel by mechanisms involving the cell membrane as well as the secretory granules.