ISOLATION AND CHARACTERIZATION OF A CDNA-ENCODING A SYNAPTONEMAL COMPLEX PROTEIN
ISOLATION AND CHARACTERIZATION OF A CDNA-ENCODING A SYNAPTONEMAL COMPLEX PROTEIN
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DOI：
10.1139/o92-147
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发表时间：
1992-10-01
期刊：
影响因子：
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通讯作者：
MOENS, PB
中科院分区：
文献类型：
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作者：
CHEN, QF;PEARLMAN, RE;MOENS, PB
用抗大鼠联会复合体蛋白的多克隆抗体筛选大鼠睾丸cDNA表达文库，克隆了编码大鼠联会复合体65千道尔顿抗原的基因SC 65。最长的开放阅读框起始于cDNA中的ATG密码子，编码431个氨基酸的蛋白质，相对分子质量为50 000。免疫分析定位SC 65基因产物的联会复合体之间的配对面平行排列的核心同源染色体在精母细胞。在检查的大鼠组织中，SC 65基因在睾丸、大脑和心脏中以相似的水平转录，而在肝脏中以低得多的水平转录。在翻译终止密码子下游延伸约80个碱基对的DNA序列与大鼠基因组中1 × 10（5）- 1.5 × 10（5）拷贝和脑特异性mRNA前体的cDNA克隆中存在的标识符序列具有93%的相似性。SC 65基因编码的氨基酸序列包含蛋白质C末端结构域中的酸性区域、潜在的糖基化位点和至少一个可能的磷酸化位点。该蛋白质与已知功能的蛋白质没有总体相似性，与GenBank或EMBL数据库中存在的蛋白质序列也没有相似性。
A gene encoding a 65-kilodalton antigen of the rat synaptonemal complex, SC65, has been cloned by screening rat testis lambdagt11 and lambdaZAPII cDNA expression libraries using polyclonal antibodies against rat synaptonemal complex proteins. The longest open reading frame, initiating at an ATG codon in the cDNA, encodes a protein of 431 amino acids, with a relative molecular mass of 50 000. Immunological analysis locates the SC65 gene product on the synaptonemal complex between the pairing faces of the parallel aligned cores of homologous chromosomes in spermatocytes. Of the rat tissues examined, the SC65 gene is transcribed in testis, brain, and heart at similar levels, and in the liver at a much lower level. The DNA sequence extending about 80 base pairs downstream of the translation termination codon has 93% similarity to the identifier sequence present in the rat genome in 1 x 10(5) - 1.5 x 10(5) copies and in cDNA clones of precursors of brain-specific mRNAs. The amino acid sequence encoded by the SC65 gene contains an acidic region in the C-terminal domain of the protein, potential glycosylation sites, and at least one possible phosphorylation site. The protein shows no overall similarity to proteins of known function, nor is there similarity to protein sequences present in GenBank or EMBL data bases.