Photoderivatized polymer thin films at quartz crystal microbalance surfaces:: Sensors for carbohydrate-protein interactions
Photoderivatized polymer thin films at quartz crystal microbalance surfaces:: Sensors for carbohydrate-protein interactions
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DOI：
10.1021/ac070740r
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发表时间：
2007-09-15
影响因子：
7.4
通讯作者：
Ramstroem, Olof
中科院分区：
文献类型：
--
作者：
Pei, Yuxin;Yu, Hui;Ramstroem, Olof
光衍生的聚合物涂层的金表面已经开发了以下的全氟苯基叠氮基的双连接策略。镀金的石英晶体微天平（QCM）晶体最初共价官能化与单层的聚（乙二醇）（PEG），使用光或热致氮烯的形成和插入。聚合物表面随后被用作底物的碳水化合物衍生的光探针的光插入，产生不同的识别图案的选择性蛋白质结合。将所得的鲁棒性和生物相容性传感器表面应用于流通式QCM仪器，用于真实的实时监测凝集素-碳水化合物相互作用。结果清楚地显示了预测的凝集素选择性，证明了该方法的适用性。
Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo-or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.