ANTIGENOMIC RNA OF HUMAN HEPATITIS DELTA-VIRUS CAN UNDERGO SELF-CLEAVAGE
ANTIGENOMIC RNA OF HUMAN HEPATITIS DELTA-VIRUS CAN UNDERGO SELF-CLEAVAGE
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DOI：
10.1128/jvi.62.8.2674-2679.1988
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发表时间：
1988-08-01
影响因子：
5.4
通讯作者：
TAYLOR, J
中科院分区：
文献类型：
--
作者：
SHARMEEN, L;KUO, MYP;TAYLOR, J
丁型肝炎病毒（HDV）的单链环状RNA基因组的结构和复制相对于已知的动物病毒是独特的，然而HDV与某些植物感染性RNA之间存在真实的相似性。因此，由于后者的一些RNA已被证明在体外进行位点特异性切割，甚至连接，我们测试的假设，类似的事件也可能发生HDV RNA。在部分证实这一假设，我们发现，在体外的RNA互补的HDV基因组，反基因组RNA，可以进行自我切割，不仅是超过90%的效率，而且只发生在一个单一的位置。发现这种切割产生与5“-羟基和环状2”，3“-单磷酸酯一致的连接片段。由于所观察到的切割都是位点特异性的，每个基因组长度只发生一次，我们建议该网站可能是相关的HDV基因组的正常细胞内复制。由于该位点几乎位于delta抗原编码区的3“末端附近，而delta抗原编码区是HDV唯一已知的功能开放阅读框架，因此我们认为切割不仅在基因组复制中起作用，而且在RNA加工中也起作用，有助于产生用于翻译delta抗原的功能性mRNA。
The structure and replication of the single-stranded circular RNA genome of hepatitis delta virus (HDV) are unique relative to those of known animal viruses, and yet there are real similarities between HDV and certain infectious RNAs of plants. Therefore, since some of the latter RNAs have been shown to undergo in vitro site-specific cleavage and even ligation, we tested the hypothesis that similar events might also occur for HDV RNA. In partial confirmation of this hypothesis, we found that in vitro the RNA complementary to the HDV genome, the antigenomic RNA, could undergo a self-cleavage that was not only more than 90% efficient but also occurred only at a single location. This cleavage was found to produce junction fragments consistent with a 5''-hydroxyl and a cyclic 2'',3''-monophosphate. Since the observed cleavage was both site-specific and occurred only once per genome length, we propose that the site may be relevant to the normal intracellular replication of the HDV genome. Because the site is located almost adjacent to the 3'' end of the delta antigen-coding region, the only known function open reading frame of HDV, we suggest that the cleavage may have a role not only in genome replication but also in RNA processing, helping to produce a functional mRNA for the translation of delta antigen.