Increased and decreased activity elicits specific morphological adaptations of the neuromuscular junction
Increased and decreased activity elicits specific morphological adaptations of the neuromuscular junction
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DOI：
10.1016/j.neuroscience.2005.10.042
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Wilson, MH
中科院分区：
文献类型：
--
作者：
Deschenes, MR;Tenny, KA;Wilson, MH
本研究的目的是比较活动减少和活动增加对神经肌肉系统的影响。选取24只7周龄的成年Sprague-Dawley大鼠，随机分为3组（N=8/组）。通过每天跑步1小时来增加活动量。后肢悬吊模型肌肉卸荷导致大鼠活动减弱。对照组为正常负重和活动的动物。在10周的干预期结束时，杀死动物，切除比目鱼肌，快速冷冻，并使用细胞荧光（神经肌肉连接）和组织化学（肌纤维）程序进行检查。通过测量神经末梢分支来量化突触前形态学，通过对终板乙酰胆碱受体进行染色来评估突触后形态学。通过测定纤维大小（横截面积）和纤维类型组成，编制了茄的肌纤维分布图。结果表明，运动训练显著提高了运动能力(P
The objective of this investigation was to compare the effects of decreased vs. increased activity on the neuromuscular system. Twenty-four young adult (7 weeks old) Sprague-Dawley rats were randomly assigned to three treatment groups (N=8/group). Increased activity was achieved by treadmill running for up to I h/day. Decreased activity was induced by muscle unloading via the hindlimb suspension model. Control animals engaged in normal weight bearing and ambulatory activity. At the end of the 10 week intervention period, animals were killed and soleus muscles were removed, quickly frozen, and examined using cytofluorescent (neuromuscular junctions) and histochemical (myofibers) procedures. Pre-synaptic morphology was quantified by measuring nerve terminal branching, and post-synaptic assessment was conducted by staining acetylcholine receptors at the endplate. Myofiber profiles of solei were compiled by determining fiber size (cross-sectional area) and fiber type composition. Results show that exercise training significantly (P