Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920
Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920
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DOI：
10.1007/s11814-009-0072-3
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发表时间：
2009-03-01
影响因子：
2.7
通讯作者：
Chen, Hsin-Liang
中科院分区：
文献类型：
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作者：
Chen, Shu-Jen;Chen, Jia-Ling;Chen, Hsin-Liang
已经探索了兽疫链球菌 ATCC 39920 生产透明质酸 (HA) 的发酵策略。 HA 比生产率 (Y-P/X) 受培养基碳氮 (C/N) 比的影响，而不是细胞比生长速率的影响。因此，HA发酵应在分批培养中最佳C/N比为2:1的平衡培养基中进行。为了提高分批培养的性能，对填充和抽取培养的操作条件进行了研究。研究发现，介质更换的时机对于成功执行填充和抽取操作至关重要。由于处于稳定期的链球菌细胞可能会失去HA合成能力，因此应在指数生长期后期开始更换填充和吸取培养物中的培养基。
The development of a fermentation strategy for hyaluronic acid (HA) production by Streptococcus zooepidemicus ATCC 39920 has been explored. The specific HA productivity (Y-P/X) was affected by the medium carbon-to-nitrogen (C/N) ratio rather than the specific growth rate of cells. Accordingly, HA fermentation should be performed in a balanced medium with an optimum C/N ratio of 2: 1 in a batch culture. To improve the performance of the batch culture, the operation conditions for the fill-and-draw culture were investigated. It was found that the timing of medium exchange is critical for successfully performing fill-and-draw operations. Since streptococcal cells at the stationary phase might lose the capacity of HA synthesis, the displacement of the medium in a fill-and-draw culture should be started at the late exponential growth phase.