Screen Printed Electrode Based Detection Systems for the Antibiotic Amoxicillin in Aqueous Samples Utilising Molecularly Imprinted Polymers as Synthetic Receptors
Screen Printed Electrode Based Detection Systems for the Antibiotic Amoxicillin in Aqueous Samples Utilising Molecularly Imprinted Polymers as Synthetic Receptors
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DOI：
10.3390/chemosensors8010005
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发表时间：
2020-03-01
期刊：
影响因子：
4.2
通讯作者：
Crapnell, Robert D.
中科院分区：
文献类型：
--
作者：
Jamieson, Oliver;Soares, Thais C. C.;Crapnell, Robert D.
合成了分子印迹聚合物（MIPs），用于选择性检测水样中的阿莫西林。不同的功能单体进行了测试，以确定最佳的组合物，通过分批再结合实验。使用相同的MIP溶液测试了两种不同的传感器平台;一种是通过将微粒滴铸到电极表面上来批量合成和表面改性的丝网印刷电极（SPE），另一种是以薄膜的形式直接UV聚合到SPE表面上。传感器用于测量阿莫西林与传热法（HTM），一种低成本和简单的热检测方法，是基于在固液界面的热阻的差异。结果表明，两种传感器平台均可检测相关浓度范围内的阿莫西林，滴铸法和直接聚合法的检测限（LOD）分别为1.89 +/- 1.03 nM和0.54 +/- 0.10 nM。利用直接UV聚合的传感器平台表现出对阿莫西林检测的增强的响应，减少的传感器制备时间和平台的选择性通过添加萘夫西林（具有相似形状和大小的药效团）来证明。MIP修饰的SPE与热检测相结合的使用提供了可用于现场分析物的快速和低成本检测的传感器，这对于环境水性样品中的污染物具有很大的潜力。平台和合成方法是通用的，并且通过调整MIP层，可以将该传感器平台扩展到各种相关目标。
Molecularly Imprinted Polymers (MIPs) were synthesised for the selective detection of amoxicillin in aqueous samples. Different functional monomers were tested to determine the optimal composition via batch rebinding experiments. Two different sensor platforms were tested using the same MIP solution; one being bulk synthesized and surface modified Screen Printed Electrodes (SPEs) via drop casting the microparticles onto the electrode surface and the other being UV polymerized directly onto the SPE surface in the form of a thin film. The sensors were used to measure amoxicillin in conjunction with the Heat-Transfer Method (HTM), a low-cost and simple thermal detection method that is based on differences in the thermal resistance at the solid-liquid interface. It was demonstrated that both sensor platforms could detect amoxicillin in the relevant concentration range with Limits of Detection (LOD) of 1.89 +/- 1.03 nM and 0.54 +/- 0.10 nM for the drop cast and direct polymerisation methods respectively. The sensor platform utilising direct UV polymerisation exhibited an enhanced response for amoxicillin detection, a reduced sensor preparation time and the selectivity of the platform was proven through the addition of nafcillin, a pharmacophore of similar shape and size. The use of MIP-modified SPEs combined with thermal detection provides sensors that can be used for fast and low-cost detection of analytes on-site, which holds great potential for contaminants in environmental aqueous samples. The platform and synthesis methods are generic and by adapting the MIP layer it is possible to expand this sensor platform to a variety of relevant targets.