AMP Aptamer Programs DNA Tile Cohesion without Canonical Base Pairing.
AMP Aptamer Programs DNA Tile Cohesion without Canonical Base Pairing.
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DOI：
10.1021/jacs.3c06260
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发表时间：
2023-08
影响因子：
15
通讯作者：
Zhe Zhang;Jin Jin-Jin;Victoria E. Paluzzi;Zhuoer Jin;Yuandong Wen;Chenghong Huang;Chun Mei Li;C. Mao;Hua Zuo
中科院分区：
文献类型：
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作者：
Zhe Zhang;Jin Jin-Jin;Victoria E. Paluzzi;Zhuoer Jin;Yuandong Wen;Chenghong Huang;Chun Mei Li;C. Mao;Hua Zuo
基于瓦片的DNA自组装为构建各种纳米结构提供了一种通用的方法，用于各种应用，如纳米医学和先进材料。瓦片间的相互作用主要通过碱基配对，特别是沃森-克里克碱基配对来编程。为了进一步扩展DNA纳米技术的工具箱，在此，我们设计了包含配体和适体的DNA瓦片。在配体-适体结合后，瓦片缔合成几何上良好限定的纳米结构。这一策略已被证明是由一系列的DNA纳米结构，其特征已被彻底的凝胶电泳和原子力显微镜组装。这种新的瓦片间的凝聚力可能会带来新的潜力，DNA自组装在未来。例如，添加游离配体可以调节纳米结构的形成。在生物配体的情况下，DNA自组装可能与某些配体的存在有关。
Tile-based DNA self-assembly provides a versatile approach for the construction of a wide range of nanostructures for various applications such as nanomedicine and advanced materials. The inter-tile interactions are primarily programmed by base pairing, particularly Watson-Crick base pairing. To further expand the tool box for DNA nanotechnology, herein, we have designed DNA tiles that contain both ligands and aptamers. Upon ligand-aptamer binding, tiles associate into geometrically well-defined nanostructures. This strategy has been demonstrated by the assembly of a series of DNA nanostructures, which have been thoroughly characterized by gel electrophoresis and atomic force microscopy. This new inter-tile cohesion could bring new potentials to DNA self-assembly in the future. For example, the addition of free ligand could modulate the nanostructure formation. In the case of biological ligands, DNA self-assembly could be related to the presence of certain ligands.