Panax ginseng ameliorates airway inflammation in an ovalbumin-sensitized mouse allergic asthma model
Panax ginseng ameliorates airway inflammation in an ovalbumin-sensitized mouse allergic asthma model
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DOI：
10.1016/j.jep.2011.04.048
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发表时间：
2011-06-14
影响因子：
5.4
通讯作者：
Yang, Woong Mo
中科院分区：
文献类型：
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作者：
Kim, Dae Yong;Yang, Woong Mo
民族药理学相关性：人参（PG）是一种草药，已被用于治疗各种免疫性疾病，包括哮喘和COPD。在这项研究中，我们研究了PG对小鼠哮喘参数的抑制机制。材料和方法：BALB/c小鼠用20 μ g/200 μ l吸附在1.0 mg/50 μ l氢氧化铝凝胶佐剂上的OVA致敏，在第0天和第14天腹腔注射。然后使用喷雾器，从第20天至第22天，每天一次用PBS中的5%OVA攻击小鼠的鼻子，持续30分钟，持续3天。在每次OVA攻击前10分钟通过腹膜内注射给予PG（20 mg/kg）或媒介物，每天一次，持续3天。测量炎性细胞向支气管肺泡灌洗液或肺组织中的募集。检测EMBP、Muc 5ac、CD 40和CD 40配体（CD 40 L）在肺组织中的表达。结果与结论：PG可恢复EMBP、Muc 5ac、CD 40和CD 40 L的表达，以及IL-1 β、IL-4、IL-5和TNF-α的mRNA和蛋白水平。另外。PG可抑制卵白蛋白致敏哮喘小鼠支气管肺泡灌洗液细胞和肺组织中杯状细胞的数量及小G蛋白和MAP激酶的增加。这些结果表明，PG可用作哮喘的治疗剂，基于各种过敏反应的减少。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Panax ginseng(PG) is a medicinal herb that has been used to treat various immune diseases including asthma and COPD. In this study, we investigated the inhibitory mechanism of PG on asthma parameters in mice.Materials and methods: BALB/c mice were sensitized with 20 mu g/200 mu l OVA adsorbed on 1.0 mg/50 mu l aluminum hydroxide gel adjuvant by i.p. injection on days 0 and 14. Mice were then challenged with 5% OVA in PBS to the nose for 30 min once a day for 3 days, from day 20 until day 22, using a nebulizer. PG (20 mg/kg) or vehicle was administrated by i.p. injection once a day 10 min before every OVA challenge for 3 days. The recruitment of inflammatory cells into bronchoalveolar lavage fluid or lung tissues was measured. The expression of EMBP, Muc5ac, CD40, and CD40 ligand (CD40L) in lung tissues was investigated. In addition, the cytokines and mitogen activated protein (MAP) kinases were measured by RT-PCR and Western blot.Results and conclusions: PG restored the expression of EMBP, Muc5ac, CD40, and CD40L, as well as the mRNA and protein levels of interleukin (IL)-1 beta, IL-4, IL-5, and tumor necrosis factor (TNF)-alpha. In addition. PG inhibited the numbers of goblet cells and further small G proteins and MAP kinases in bronchoalveolar lavage cells and lung tissues increased in ovalbumin-induced allergic asthma in mice. These results suggest that PG may be used as a therapeutic agent in asthma, based on reductions of various allergic responses. (C) 2011 Elsevier Ireland Ltd. All rights reserved.