Structural insights into the classification of Mud Crab Reovirus
Structural insights into the classification of Mud Crab Reovirus
复制标题
泥蟹呼肠孤病毒分类的结构见解
DOI：
10.1016/j.virusres.2012.02.025
复制
发表时间：
2012-06-01
期刊：
影响因子：
5
通讯作者：
Weng, Shaoping
中科院分区：
文献类型：
--
作者：
Huang, Zengwei;Deng, Xiexiong;Weng, Shaoping
应用冷冻电镜技术对引起锯缘青蟹“昏睡病”的青蟹呼肠孤病毒（MCRV）进行了研究。我们在这里提出的MCRV在13.8埃分辨率的三维结构。外壳由260个三聚体组成，具有完全的T = 13二十面体对称性。MCRV与先前报道的水生呼肠孤病毒之间的主要区别在于它缺乏五聚体的转塔结构。这些结果连同最近发表的分子生物学证据（Deng等人，2012）表明，从结构的角度来看，MCRV应被分类为呼肠孤病毒科的新成员。(C)2012爱思唯尔有限公司版权所有。
Cryo-electron microscopy was applied to analyze mud crab reovirus (MCRV), which causes 'sleeping disease' in mud crab, Scylla serrata, a marine species cultured in China. We present here the three dimensional structure of MCRV at 13.8 angstrom resolution. The outer capsid shell is composed of 260 trimers with complete T = 13 icosahedral symmetry. A major difference between MCRV and previously reported aquareoviruses is that it lacks a pentameric turret structure. These results together with recently published molecular biological evidence (Deng et al., 2012) indicate that, from a structural perspective, MCRV should be classified as a new member of the family Reoviridae. (C) 2012 Elsevier B.V. All rights reserved.