An interlaboratory study of perfluorinated alkyl compound levels in human plasma
An interlaboratory study of perfluorinated alkyl compound levels in human plasma
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DOI：
10.1016/j.envres.2008.01.005
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发表时间：
2008-06-01
影响因子：
8.3
通讯作者：
Studabaker, William B.
中科院分区：
文献类型：
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作者：
Longnecker, Matthew P.;Smith, Cynthia S.;Studabaker, William B.
我们进行了一项不同于这类典型研究的实验室间研究，因为它强调比较结果的实验室内可变性。我们将样本送往六个在血液基质中全氟烷基化合物分析方面经验丰富的实验室，这些实验室使用严格的程序来控制和确保准确度和精密度。每个人都接受了一套相同的60个血浆样本，这些样本被分成六个完全独立的批次进行分析。分离的样品被包括在内，以便可以计算批内和批间的变异系数。所有实验室均采用液质联用(LC-MS/MS)。在样品中测量的全氟辛烷磺酸(PFOS)、全氟辛酸(PFOA)和全氟己烷磺酸(PFHxS)的浓度总体上显示出很高的一致性，尽管在某些情况下只是中等程度的一致性。全氟辛烷磺酸的批内和批间平均变异系数分别为9.1%和9.3%；全氟辛烷磺酸的批内和批间平均变异系数分别为14.5%和14.5%；全氟辛烷磺酸的批内和批间变异系数分别为14.5%和17.0%。最近标记的内标的可获得性，以及其他方面的进展，促进了分析准确度和精密度的提高。考虑到背景暴露人群中受试者之间水平的差异程度，结果表明，基于生物标记物的与健康相关的流行病学研究可能具有合理的精确度。(C)2008 Elsevier Inc.保留所有权利。
We conducted an interlaboratory study which differed from the typical study of this type because of its emphasis on comparing intralaboratory variability in results. We sent specimens to six laboratories experienced in the analysis of perfluorinated alkyl compounds in blood matrices and that use stringent procedures to control and assure accuracy and precision. Each received an identical set of 60 plasma specimens that were analyzed in six completely independent batches. Split specimens were included so that within- and between-batch coefficients of variation could be calculated. All laboratories used liquid chromatography-tandem mass spectrometry (LC-MS/MS). The concentrations of perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), and perfluorohexanesulfonate (PFHxS) measured in the specimens in general showed a high level of agreement, although in some cases the agreement was only moderate. The average within- and between-batch coefficient of variation for PFOS was 9.1% and 9.3%; for PFOA was 14.5% and 14.5%; and for PFHxS was 14.5% and 17.0%. The recent availability of labeled internal standards, among other advances, has facilitated improvement in the accuracy and precision of the assays. Considering the degree of between-subject variation in levels among people in background-exposed populations, the results indicate that biomarker-based epidemiologic studies of associations with health could have reasonable precision. (C) 2008 Elsevier Inc. All rights reserved.