PbrPCCP1 mediates the PbrTTS1 signaling to control pollen tube growth in pear
PbrPCCP1 mediates the PbrTTS1 signaling to control pollen tube growth in pear
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PbrPCCP1 介导 PbrTTS1 信号控制梨花粉管生长
DOI：
10.1016/j.plantsci.2019.110244
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Zhang ShaoLing
中科院分区：
文献类型：
--
作者：
Jiao HuiJun;Liu Qian;Zhang Hao;Qi Kaijie;Liu Zhe;Wang Peng;Wu JuYou;Zhang ShaoLing
在植物中，含有C2结构域的基因已被鉴定，并在许多关键生理过程中起着至关重要的作用。在梨中鉴定出166个含有C2结构域的基因，38个含有多个C2结构域的基因。全基因组重复和串联重复事件是驱动C2超家族扩张的主要力量，C2超家族成员在负选择下进化。在花粉管生长过程中有104个C2基因表达。在此，我们从梨中鉴定出含有C2结构域的Pbr028378.1，并将其命名为PbrPCCP 1。PbrPCCP 1定位于质膜，主要在花粉中表达。PbrPCCP 1与PbrTTS 1相互作用，PbrTTS 1含有富含Cys的C端结构域，并促进花粉管生长。PbrTTS 1的Pollen ole e I结构域负责其相互作用。此外，当PbrPCCP 1表达水平被反义寡核苷酸敲低时，花粉管生长受到抑制，PbrTTS 1的促进作用减弱。qRT-PCR结果表明，PbrPCCP 1和PbrTTS 1在授粉后花柱中的表达水平一致，且在授粉后24 h内表达水平上调。这表明，当花粉管在雌蕊中生长时，它们可以共同调节花粉管的生长。
In plants, genes containing the C2 domain have been identified and play a crucial role in many key physiological processes. One hundred and sixty-six genes containing a C2 domain were identified in pear and 38 genes contained multiple C2 domains. Whole genome duplication and tandem duplication events were the major forces driving the C2 superfamily expansion, and C2 superfamily members have evolved under negative selection. There were 104 C2 genes expressed during pollen tube growth. Here, we identifiedPbr028378.1containing the C2 domain from pear and named itPbrPCCP1. PbrPCCP1 was localized in the plasma membrane and mainly expressed in pollen. PbrPCCP1 interacted with PbrTTS1, which contained a Cys-rich C-terminal domain, and promoted pollen tube growth. The Pollen ole e I domain of PbrTTS1 was responsible for its interaction. Additionally, pollen tube growth was inhibited and the promoting effect of PbrTTS1 was attenuated whenPbrPCCP1expression level was knocked-down by antisense oligonucleotides. The qRT-PCR results indicated thatPbrPCCP1andPbrTTS1expression levels were consistently present in the style after pollination, and their expression levels were up-regulated within 24 h. This implied that they could co-regulate pollen tube growth when the pollen tube grew in the pistil.