Production of Butanol Directly from Hemicellulose through Secretory Expression of a Xylanase in Clostridium acetobutylicum
Production of Butanol Directly from Hemicellulose through Secretory Expression of a Xylanase in Clostridium acetobutylicum
复制标题
通过丙酮丁醇梭菌中木聚糖酶的分泌表达直接从半纤维素生产丁醇
DOI：
10.1021/acs.energyfuels.9b04489
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发表时间：
2020
期刊：
影响因子：
5.3
通讯作者：
Ying Hanjie
中科院分区：
文献类型：
--
作者：
Wang Zhenyu;Cao Xingyuan;Li Nan;Yang Zhengjiao;Lei Ming;Zhao Yang;Wang Lizhi;Li Zhenyu;Liu Dong;Niu Huanqing;Ying Hanjie
丁醇是一种极具吸引力的第二代生物燃料，也是一种重要的化工产品。近年来，通过整合的生物加工从木质纤维素原料生产丁醇引起了越来越多的兴趣。然而，这受到丁醇生产菌株中胞外多糖降解效率低下的限制。在这项研究中，生产丁醇直接从半纤维素简单地实现了通过过表达的土著木聚糖酶在丙酮丁醇梭菌。CA_P0053编码的木聚糖酶（XynB）具有高可溶性，并能从C.丙酮丁醇胞外木聚糖酶活性提高了88倍，从半纤维素中获得4.03 g/L的生物丁醇，这是迄今为止报道的从半纤维素或木聚糖中获得的最高产量。本研究证明了产溶剂C.丙酮丁醇在联合生产生物燃料方面具有巨大潜力。了解和应用其分泌机制将具有重要意义。
Butanol is an attractive second-generation biofuel as well as an important chemical. Production of butanol from lignocellulosic feedstock via consolidated bioprocessing has attracted increasing interest in recent years. However, this has been limited by the inefficient degradation of extracellular polysaccharides in butanol-producing strains. In this study, production of butanol directly from hemicellulose was achieved simply through overexpression of an indigenous xylanase in Clostridium acetobutylicum. The xylanase (XynB) encoded by CA_P0053 was demonstrated highly soluble and fully secreted from C. acetobutylicum. The extracellular xylanase activity was increased 88-fold and 4.03 g/L of biobutanol was obtained from hemicellulose, which was the highest yield yet reported from either hemicellulose or xylan. This study proved that the solventogenic C. acetobutylicum had great potential for consolidated production of biofuels. Understanding and applying its secretion mechanism will be of great significance...