Molecular and morphological evidence of hybridization between native Ruditapes philippinarum and the introduced Ruditapes form in Japan
Molecular and morphological evidence of hybridization between native Ruditapes philippinarum and the introduced Ruditapes form in Japan
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DOI：
10.1007/s10592-013-0467-x
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发表时间：
2013-06-01
影响因子：
2.2
通讯作者：
Kishino, Hirohisa
中科院分区：
文献类型：
--
作者：
Kitada, Shuichi;Fujikake, Chie;Kishino, Hirohisa
海洋水产养殖和种群增殖是引进外来物种的主要原因。这种引进的一个很好的例子是日本短颈蛤，世界上最重要的渔业资源之一。为解决国内R.由于捕捞量减少而引起的菲律宾蛤仔病，日本从中国和朝鲜半岛进口蛤仔。进口的文蛤是一种外来种，形态非常相似，被误认为是R。菲律宾蛤仔（Ruditapes form）。我们对1,186只蛤进行了基因分型。philippinarum和R.在4个微卫星位点上测定了485个文蛤的线粒体DNA（COI基因片段），其中34个为文蛤。variegatus，并测量了来自日本和中国的12个群体的754个蛤的形态和分裂特征，包括有明海和东京湾，其中大量的R.表格已发布。我们的分析证实了R.型来自蛤仔属，并讨论了R. philippinarum和R.形式是不同的，但小，相比，五个双壳类外类群。然而，R.型的壳形态特征明显，特别是壳表面有较多的放射状肋，表明R.形式可能是一个新的Ruditapes种或R.原产于中国南方。有明海样品与R.在有明海中，以中间壳的形态和辐射肋的数量为特征，推测其杂种比例为51.3 +/- A 4.6%。
Marine aquaculture and stock enhancement are major causes of the introduction of alien species. A good example of such an introduction is the Japanese shortneck clam Ruditapes philippinarum, one of the most important fishery resources in the world. To meet the domestic shortage of R. philippinarum caused by depleted catches, clams were imported to Japan from China and the Korean peninsula. The imported clam is an alien species that has a very similar morphology, and was misidentified as R. philippinarum (hereafter, Ruditapes form). We genotyped 1,186 clams of R. philippinarum and R. form at four microsatellite loci, sequenced mitochondrial DNA (COI gene fragment) of 485 clams, 34 of which were R. variegatus, and measured morphometric and meristic characters of 754 clams from 12 populations in Japan and China, including the Ariake Sea and Tokyo Bay, where large numbers of R. form were released. Our analyses confirmed that R. form was from the genus Ruditapes, and the genetic differentiation between R. philippinarum and R. form was distinct, but small, compared with five bivalve outgroups. However, R. form had distinct shell morphology, especially larger numbers of radial ribs on the shell surface, suggesting that R. form might be a new Ruditapes species or a variation of R. philippinarum that originated from southern China. A genetic affinity of the sample from the Ariake Sea to R. form was found with the intermediate shell morphology and number of radial ribs, and the hybrid proportion was estimated at 51.3 +/- A 4.6 % in the Ariake Sea.