Comparing behavioral responses across multiple assays of stress and anxiety in zebrafish (Danio rerio)
Comparing behavioral responses across multiple assays of stress and anxiety in zebrafish (Danio rerio)
复制标题
比较斑马鱼（Danio rerio）多种压力和焦虑测定的行为反应
DOI：
10.1163/1568539x-00003018
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发表时间：
2012
期刊：
影响因子：
1.3
通讯作者：
J. Godwin
中科院分区：
文献类型：
--
作者：
Ryan Y. Wong;Florence Perrin;Sarah E. Oxendine;Zachary D. Kezios;Susanna Sawyer;Linran Zhou;Simone Dereje;J. Godwin
动物一生中会多次遇到压力情况，并且通常都能成功应对。个体对压力源的行为和生理反应的性质和强度各不相同，通常代表相关且性质不同的应对方式（例如主动和被动）。这些替代的应对方式是动物克服各种压力情况的方法，最终会产生重要的健康后果。在这里，我们使用最近野生的斑马鱼（斑马鱼）和选择性繁殖的静态行为（高线和低线）和经典的驯化品系（AB）来记录这些斑马鱼品系在理解应对机制方面的效用。在六种与压力和焦虑相关的行为测定中，斑马鱼的低稳态行为 (LSB) 系表现出明显低于高稳态行为 (HSB) 的压力和焦虑相关行为。在一些测定中，我们在试验开始后三分钟内观察到菌株行为差异。在两项检测中，相对于女性，男性的焦虑相关行为水平也有所降低。比较野生菌株和驯化菌株，AB 系在一半的检测中表现出明显较低水平的焦虑相关行为。这项研究表明，我们从野生捕获的斑马鱼（HSB、LSB）中选择性培育的品系在多个不同的测定中表现出一致和不同的行为应激反应。因此，这些线条可能有助于理解应对压力和焦虑的直接和最终机制。
Animals encounter stressful situations multiple times throughout their lives and often successfully cope with them. Individuals vary in the nature and intensity of their behavioral and physiological response to stressors, often representing correlated and qualitatively distinct coping styles (e.g., proactive and reactive). These alternative coping styles are ways an animal can overcome a variety of stressful situations, which ultimately can have important fitness consequences. Here we use zebrafish (Danio rerio) recently wild-derived and selectively bred for amount of stationary behavior (High and Low lines) and a classic domesticated strain (AB) to document the utility of these zebrafish strains in understanding coping mechanisms. The Low Stationary Behavior (LSB) line of zebrafish displayed significantly lower stress and anxiety-related behaviors than the High Stationary Behavior (HSB) across six stress and anxiety-related behavioral assays. In some assays, we observed strain differences in behavior within three minutes of the start of the trial. Males also showed reduced levels of anxiety-related behaviors relative to females in two assays. Comparing wild-derived and domesticated strains, the AB line displayed significantly lower levels of anxietyrelated behavior in half of the assays. This study demonstrates that our selectively bred lines from wild-caught zebrafish (HSB, LSB) exhibit consistent and divergent behavioral stress responses across multiple distinct assays. Hence these lines may prove useful in understanding the proximate and ultimate mechanisms of coping with stress and anxiety.
影响因子：
2
作者：
Bill BR;Petzold AM;Clark KJ;Schimmenti LA;Ekker SC
通讯作者：
Ekker SC