Porcine Hepcidin Exerts an Iron-Independent Bacteriostatic Activity Against Pathogenic Bacteria
Porcine Hepcidin Exerts an Iron-Independent Bacteriostatic Activity Against Pathogenic Bacteria
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猪铁调素对病原菌具有不依赖于铁的抑菌活性
DOI：
10.1007/s10989-014-9451-7
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发表时间：
2015-06
影响因子：
2.5
通讯作者：
Du, Huahua
中科院分区：
文献类型：
--
作者：
Pu, Yutian;Xiong, Haitao;Wang, Yizhen;Du, Huahua
海普西丁最初被鉴定为一种抗菌肽，后来证明海普西丁是长期寻求的调节哺乳动物体内铁稳态的激素。虽然它的铁调节功能已被广泛研究，但对其抗菌性能的研究还很有限。本研究的目的是通过放射状扩散法、菌落形成计数法、透射电子显微镜和DNA结合试验来评价合成的猪海普西丁(PHepc)对病原菌的体外抗菌活性。结果表明，pHepc对大肠埃希菌K88、大肠埃希菌ATCC 25922、金黄色葡萄球菌ATCC 25923和鼠伤寒沙门氏菌CMCC 50013均有一定的抑菌作用，但杀菌活性较弱。PHepc-治疗的E。ColiK88细胞较长，胞质分布不均匀，而pHepc处理组S细胞较长。出现细胞质渗漏和细菌细胞部分溶解。凝胶滞留实验表明，pHepc与DNA存在结合亲和力。此外，pHepc在pH值为4.0~8.0的范围内和在有铁存在的情况下都保持了抑菌活性。考虑到PHepc在感染后的高表达和抑菌活性，PHepc可能是猪健康的重要防御分子。
Hepcidin was first identified as an antimicrobial peptide and later demonstrated that hepcidin is the long sought hormone to regulate iron homeostasis in mammals. Though its iron regulatory function has been extensively investigated, the studies on its antimicrobial properties are limited. The aim of current study was to evaluate the antibacterial activity of synthetic porcine hepcidin (pHepc) in vitro against pathogen bacteria via radial diffusion, colony forming count, transmission electron microscopy and DNA binding assays. Our results showed that pHepc exerted little bactericidal activity, but possessed bacteriostatic activity by reducing the viableEscherichia coliK88,E. coliATCC 25922,Staphylococcus aureusATCC 25923 andSalmonella typhimuriumCMCC 50013. pHepc-treatedE. coliK88 exhibited longer cells and cytoplasm unevenly distribution, while pHepc-treatedS. aureusled to cytoplasm leakage and partly lysis of bacterial cells. Gel retardation assay showed the existence of the binding affinity of pHepc for DNA. In addition, pHepc retained the bacteriostatic activity in a wide range of pH value from 4.0 to 8.0 or in the presence of iron, respectively. Considering the high expression in response to infection and the bacteriostatic activity, pHepc may be an important defense molecule for pig health.
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