Laser desorption ionization of red phosphorus clusters and their use for mass calibration in time-of-flight mass spectrometry
Laser desorption ionization of red phosphorus clusters and their use for mass calibration in time-of-flight mass spectrometry
复制标题
DOI：
10.1002/rcm.4230
复制
发表时间：
2009-10-01
影响因子：
2
通讯作者：
Havel, Josef
中科院分区：
文献类型：
--
作者：
Sladkova, Katerina;Houska, Jan;Havel, Josef
红磷通过激光解吸电离(LDI)很容易形成P-n(n=1-)团簇，其覆盖范围可达约M/z 3000，正负离子模式均可。这些团簇是单电荷的，光谱很简单，因为磷是单同位素的。质谱图可以用可接受的分辨率和强度进行测量。研究了利用带正电荷的P-n团簇进行质谱学定标的方法，结果表明，在外部定标中，即使使用普通的商用基质辅助激光解吸电离飞行时间(MALDI-TOF)仪器，也可以获得+/-0.04m/z单位的标准偏差。当用作内标时，P-n簇与一些分析物--例如C-60和C-70富勒烯和葫芦脲[8]发生反应。还发现红磷是多肽和蛋白质的合适的MALDI基质，以缓激肽、血管紧张素、肾素、促肾上腺皮质激素ACTH片段18-359和胰岛素以及单独的胰岛素的CalMix为例。版权所有(C)2009 John Wiley&Sons，Ltd.
Phosphorus clusters P-n (n = 1-89) are easily formed from red phosphorus by laser desorption ionization (LDI) and they cover a range of up to approx. m/z 3000 in both positive and negative ion mode. The clusters are singly charged and the spectra are simple because phosphorus is monoisotopic. The mass spectra can be measured with an acceptable resolution and intensity. The use of positively charged P-n clusters for calibration in mass spectrometry was examined and it was demonstrated that in external calibration a standard deviation of +/-0.04 m/z units can be achieved even when using a common commercial matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrument. When used as internal standards the P-n clusters react with some analytes - C-60 and C-70 fullerenes and cucurbituril[8], for example. It was also found that red phosphorus is a suitable MALDI matrix for peptides and proteins, illustrated by the examples of a Calmix mixture of bradykinin, angiotensin, renin, adrenocorticotropic hormone ACTH fragment 18-359 and insulin, and of insulin alone. Copyright (C) 2009 John Wiley & Sons, Ltd.