Silk fibroin/chitosan scaffold with tunable properties and low inflammatory response assists the differentiation of bone marrow mesenchymal stem cells
Silk fibroin/chitosan scaffold with tunable properties and low inflammatory response assists the differentiation of bone marrow mesenchymal stem cells
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DOI：
10.1016/j.ijbiomac.2017.07.080
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发表时间：
2017-12-01
影响因子：
8.2
通讯作者：
Yin, Da-Chuan
中科院分区：
文献类型：
--
作者：
Li, Da-Wei;Lei, Xiaohua;Yin, Da-Chuan
支架的物理和化学性质在三维（3D）细胞培养中起着重要作用，它总是决定细胞的命运或植入的结果。控制这些特性对于满足各种组织工程应用的要求是必要的。本研究采用冷冻干燥法制备了一系列性能可调的丝素/壳聚糖（SF/CS）支架，并将大鼠骨髓间充质干细胞（bmscs）植入支架中，以评价其在组织工程中的应用价值。通过改变前驱体溶液的总浓度和SF与CS的混合比例，可以调节SF/CS支架的三维结构、力学性能和降解能力。在SF/CS支架中培养的BM-MSCs具有良好的增殖能力和多种形态。体外培养时，骨髓间充质干细胞成功诱导成骨分化和成脂分化。SF/CS支架皮下植入4周内未发生任何炎症反应，相容性良好。此外，植入的支架允许宿主细胞侵入、粘附、生长并形成新的血管。由于这些优异的性能，SF/CS支架在制备组织工程植入物方面具有很大的潜力。(C) 2017年Elsevier B.V.出版
The physical and chemical properties of the scaffold are known to play important roles in three-dimensional (3D) cell culture, which always determine the cellular fate or the results of implantation. To control these properties becomes necessary for meeting the requirements of a variety of tissue engineering applications. In this study, a series of silk fibroin/chitosan (SF/CS) scaffolds with tunable properties were prepared using freeze-drying method, and the rat bone marrow-derived mesenchymal stem cells (BM-MSCs) were seeded in these scaffolds to evaluate their availability of use in tissue engineering. The 3D structure, mechanical properties and degradation ability of SF/CS scaffold can be tuned by changing the total concentration of the precursor solution and the blending ratio between SF and CS. BM-MSCs cultured in'the SF/CS scaffold exhibited excellent proliferation and multiple morphologies. The induction of osteogenic and adipogenic differentiation of BM-MSCs were successful in this scaffold when cultured in vitro. Subcutaneous implantation of the SF/CS scaffolds did not cause any inflammatory response within four weeks, which revealed good compatibility. Moreover, the implanted scaffold allowed host cells to invade, adhere, grow and form new blood vessels. With these excellent performance, SF/CS scaffold has great potential in preparing implants for tissue engineering applications. (C) 2017 Published by Elsevier B.V.