Cell type-specific phosphorylation and proteolysis of a transcriptional regulator controls the G1-to-S transition in a bacterial cell cycle
Cell type-specific phosphorylation and proteolysis of a transcriptional regulator controls the G1-to-S transition in a bacterial cell cycle
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DOI：
10.1016/s0092-8674(00)80502-4
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发表时间：
1997-08-08
期刊：
影响因子：
64.5
通讯作者：
Shapiro, L
中科院分区：
文献类型：
--
作者：
Domian, IJ;Quon, KC;Shapiro, L
全局性转录调控因子CtrA控制着Caulbacter细胞周期中的多个事件，包括DNA复制的启动、DNA甲基化、细胞分裂和鞭毛生物发生。CTRA是双组分信号转导系统中反应调节因子家族的一员，被磷酸化激活。我们在这里报告了这种磷酸化信号在S中期进入细胞周期。此外，CtrA的功能受时间和空间控制的蛋白降解的调节。当活性的CtrA蛋白出现在细胞周期的错误时间时，由于突变的CtrA衍生物的表达在没有磷酸化的情况下是活跃的，并且在细胞周期中不被翻转，从G1到S的转变被阻止，细胞周期中止。因此，磷酸化和蛋白分解都是细菌细胞周期控制的关键决定因素，其方式类似于真核细胞周期的控制。
The global transcriptional regulator CtrA controls multiple events in the Caulobacter cell cycle, including the initiation of DNA replication, DNA methylation, cell division, and flagellar biogenesis. CtrA is a member of the response regulator family of two component signal transduction systems and is activated by phosphorylation. We report here that this phosphorylation signal enters the cell cycle at mid S phase. In addition, CtrA function is modulated by temporally and spatially controlled proteolysis. When an active CtrA protein is present at the wrong time in the cell cycle, owing to expression of a mutant CtrA derivative that is active in the absence of phosphorylation and is not turned over during the cell cycle, the G1-to-S transition is blocked and the cell cycle aborts. Thus, both phosphorylation and proteolysis are critical determinants of bacterial cell cycle control in a manner that is analogous to the control of the eukaryotic cell cycle.