Size-Dependent Cytotoxicity and Reactive Oxygen Species of Cerium Oxide Nanoparticles in Human Retinal Pigment Epithelia Cells.
Size-Dependent Cytotoxicity and Reactive Oxygen Species of Cerium Oxide Nanoparticles in Human Retinal Pigment Epithelia Cells.
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DOI：
10.2147/ijn.s305676
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发表时间：
2021
影响因子：
8
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Ma Y;Li P;Zhao L;Liu J;Yu J;Huang Y;Zhu Y;Li Z;Zhao R;Hua S;Zhu Y;Zhang Z
氧化铈纳米颗粒（CeO 2 NPs）是一种镧系元素氧化物和二价化合物，在工业和生物医学中的应用不断增长。由于其广泛的应用，CeO 2纳米颗粒潜在的人类健康问题引起了人们的关注，但缺乏关于该化合物对人眼毒性的研究。本研究研究了CeO 2纳米颗粒在人视网膜色素上皮细胞（ARPE-19细胞）中的细胞毒性和活性氧（ROS）。利用透射电子显微镜（TEM）对CeO 2纳米粒子的粒径分布和形貌进行了表征。为探讨CeO 2纳米粒粒径对眼毒性的影响，采用ATP含量测定、LDH释放测定和细胞增殖测定等方法研究了15、30和45 nm三种粒径CeO 2纳米粒对ARPE-19细胞的毒性作用。用H2 DCF-DA染色和JC-1染色检测细胞内ROS含量和线粒体膜电位去极化。使用相差显微镜检测形态学变化。发现15 nm CeO 2纳米颗粒的细胞毒性最高，因此进一步探索。用15 nm CeO 2 NPs处理导致ARPE-19细胞的形态以剂量和时间依赖性方式改变。此外，处理诱导过量的ROS产生和线粒体膜电位去极化。此外，通过应用ROS清除剂N-乙酰基-L-半胱氨酸（NAC）来减弱细胞毒性。CeO 2 NPs在ARPE-19细胞中诱导细胞毒性和ROS的过度产生以及线粒体膜电位的降低。ROS的过度产生部分地有助于CeO 2 NP诱导的细胞毒性。
The use of cerium oxide nanoparticles (CeO2 NPs), a lanthanide element oxide and bivalent compound, has been growing continuously in industry and biomedicine. Due to their wide application, the potential human health problems of CeO2 NPs have attracted attention, but studies on the toxicity of this compound to human eyes are lacking. This study investigated the cytotoxicity and reactive oxygen species (ROS) of CeO2 NPs in human retinal pigment epithelial cells (ARPE-19 cells). Using the transmission electron microscope (TEM), the size distribution and shape of CeO2 NPs were characterized. To explore the effect of CeO2 NP size on ophthalmic toxicity in vitro, three sizes (15, 30 and 45 nm) of CeO2 NPs were investigated using ATP content measurement, LDH release measurement and cell proliferation assay in ARPE-19 cells. ROS values and mitochondrial membrane potential depolarization were evaluated by H2DCF-DA staining and JC-1 staining. Morphology changes were detected using a phase-contrast microscope. The cytotoxicity of 15 nm CeO2 NPs was found to be the highest and hence was further explored. Treatment with 15 nm CeO2 NPs caused the morphology of ARPE-19 cells to change in a dose- and time-dependent manner. Moreover, the treatment induced excessive ROS generation and mitochondrial membrane potential depolarization. In addition, cytotoxicity was attenuated by the application of a ROS scavenger N-acetyl-L- cysteine (NAC). CeO2 NPs induced cytotoxicity in ARPE-19 cells and excessive production of ROS and decreasing mitochondrial membrane potential. The Overproduction of ROS partially contributes to CeO2 NP-induced cytotoxicity.