CELL-SPECIFIC EXPRESSION OF THE RAT INSULIN GENE - EVIDENCE FOR ROLE OF 2 DISTINCT-5' FLANKING ELEMENTS
CELL-SPECIFIC EXPRESSION OF THE RAT INSULIN GENE - EVIDENCE FOR ROLE OF 2 DISTINCT-5' FLANKING ELEMENTS
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DOI：
10.1126/science.3904002
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
RUTTER, WJ
中科院分区：
文献类型：
--
作者：
EDLUND, T;WALKER, MD;RUTTER, WJ
大鼠胰岛素I基因的5‘侧翼DNA包含控制细胞特异性表达的序列。通过用病毒系统中的非歧视控制元件替换特定部分对该区域的分析表明，转录增强子位于5‘侧翼DNA的末端；其位置已通过缺失分析进行了定位。其他实验表明，另一个不同的调控元件位于转录起始点附近。这两种成分的活性仅限于胰腺B细胞。多种调控元件的联合作用可以解释胰岛素基因的细胞特异性表达。
The 5′ flanking DNA of the rat insulin I gene contains sequences controlling cell-specific expression. Analysis of this region by replacement of specific portions with nondiscriminatory control elements from viral systems shows that a transcriptional enhancer is located in the distal portion of the 5′ flanking DNA; its position has been mapped by deletion analysis. Additional experiments suggest that another distinct regulatory element is located more proximal to the transcription start site. The activity of both elements is restricted to pancreatic B cells. The combinatorial effect of multiple control elements could explain the cell-specific expression of insulin genes.