Comparative Metabolomics Revealed the Potential Antitumor Characteristics of Four Endophytic Fungi of Brassica rapa L.
Comparative Metabolomics Revealed the Potential Antitumor Characteristics of Four Endophytic Fungi of Brassica rapa L.
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DOI：
10.1021/acsomega.9b04258
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发表时间：
2020-03
期刊：
影响因子：
4.1
通讯作者：
Jie Wei;Feng Chen;Yumei Liu;Azierguli Abudoukerimu;Qian Zheng;Xiaobo Zhang;Yuping Sun;Delixiati Yimiti
中科院分区：
文献类型：
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作者：
Jie Wei;Feng Chen;Yumei Liu;Azierguli Abudoukerimu;Qian Zheng;Xiaobo Zhang;Yuping Sun;Delixiati Yimiti
药用植物内生真菌因其具有多种与寄主植物相似的活性生化物质、易于发酵和培养而受到广泛关注。白菜作为新疆传统的药食同源植物，应用历史悠久。最近，研究表明白菜具有降血糖、抗菌、免疫调节和抗氧化特性。然而，目前尚未对白菜内生真菌的功能和多样性进行研究。本实验室从白菜内生真菌中分离得到4株内生真菌（pr6、pr7、pr8和pr10）。 pr10 的代谢提取物具有显着的抗肿瘤活性。本研究通过比较代谢组学分析，从种类和含量上，与其他三种内生真菌相比，确定了pr10的优势代谢物。代谢组学分析结果表明，pr10的代谢产物富含氨基酸和海藻糖等糖衍生物，其对A549细胞系的抑制能力已被证明。本研究为开发利用白菜及其内生真菌形成抗肿瘤药物提供理论依据。
Endophytic fungi of medicinal plants have attracted wide attention due to their various active biochemical substances that are similar to those of the host plants and can be easily fermented and cultured. As a traditional medicine and food homologous plant in Xinjiang, Brassica rapa L. has a long history of applications. Recently, it has been shown that B. rapa L. has hypoglycemic, antimicrobial, immunomodulatory, and antioxidant properties. However, there are no studies on the function and diversity of enophytic fungi of B. rapa L. Four endophytic fungus (pr6, pr7, pr8, and pr10) strains were isolated from B. rapa L. in our laboratory. The metabolic extracts from pr10 have significant effects in terms of antitumor activity. In this study, in terms of types and contents, compared with those of the other three endophytic fungi, the dominant metabolites of pr10 were determined by comparative metabolomics analysis. The results of metabolomics analysis indicated that the metabolites of pr10 are rich in amino acids and sugar derivatives such as trehalose, whose ability to inhibit the A549 cell line has been proved. This study provides a theoretical basis for the development and utilization of B. rapa L. and its endophytic fungi to form antitumor agents.