Dynamic Plastid Redox Signals Integrate Gene Expression and Metabolism to Induce Distinct Metabolic States in Photosynthetic Acclimation in Arabidopsis
Dynamic Plastid Redox Signals Integrate Gene Expression and Metabolism to Induce Distinct Metabolic States in Photosynthetic Acclimation in Arabidopsis
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DOI：
10.1105/tpc.108.062018
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发表时间：
2009-09-01
期刊：
影响因子：
11.6
通讯作者：
Pfannschmidt, Thomas
中科院分区：
文献类型：
--
作者：
Braeutigam, Katharina;Dietzel, Lars;Pfannschmidt, Thomas
植物具有适应性反应，在这种反应中，光合机构的结构重构使其适应光照的波动。长期驯化涉及质体和核基因表达的变化，并由光合作用的氧化还原信号控制。这些信号的动力学和能量和代谢需求的光合机构的变化的调整目前知之甚少。使用氧化还原信号系统，优先激发光系统I或II，我们测量了时间依赖性的影响，氧化还原信号的转录组和代谢组的拟南芥。我们观察到快速和动态的变化，核转录本积累导致差异和特定的表达模式与光合作用和代谢相关的基因。代谢物池也表现出动态变化，并表明不同的代谢状态之间的重新调整，这取决于各自的照明。这些状态反映了能量资源的重新分配在一个定义和可逆的方式，表明在长期驯化过程中的光合机构的结构变化，另外支持在代谢水平。我们认为，光合作用可以作为一个环境传感器，产生逆行氧化还原信号，触发两个平行的调节回路，协调光合作用和代谢，以适应植物初级生产力的环境。
Plants possess acclimation responses in which structural reconfigurations adapt the photosynthetic apparatus to fluctuating illumination. Long-term acclimation involves changes in plastid and nuclear gene expression and is controlled by redox signals from photosynthesis. The kinetics of these signals and the adjustments of energetic and metabolic demands to the changes in the photosynthetic apparatus are currently poorly understood. Using a redox signaling system that preferentially excites either photosystem I or II, we measured the time-dependent impact of redox signals on the transcriptome and metabolome of Arabidopsis thaliana. We observed rapid and dynamic changes in nuclear transcript accumulation resulting in differential and specific expression patterns for genes associated with photosynthesis and metabolism. Metabolite pools also exhibited dynamic changes and indicate readjustments between distinct metabolic states depending on the respective illumination. These states reflect reallocation of energy resources in a defined and reversible manner, indicating that structural changes in the photosynthetic apparatus during long-term acclimation are additionally supported at the level of metabolism. We propose that photosynthesis can act as an environmental sensor, producing retrograde redox signals that trigger two parallel adjustment loops that coordinate photosynthesis and metabolism to adapt plant primary productivity to the environment.