Genomic sequence of a 12S seed storage protein from oilseed rape (Brassica napus c.v. jet neuf).

Genomic sequence of a 12S seed storage protein from oilseed rape (Brassica napus c.v. jet neuf).
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来自油菜 (Brassica napus c.v. jet neuf) 的 12S 种子储存蛋白的基因组序列。

DOI:
10.1093/nar/17.9.3584
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发表时间:
1989
影响因子:
14.9
通讯作者:
Charles H. Shaw
Charles H. Shaw
中科院分区:
生物学2区
文献类型:
--
作者:
Anderson J. Ryan;Christopher L. Royal;Joe Hutchinson;Charles H. Shaw

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含有胚胎特异性储存蛋白十字花科素完整基因的克隆已从甘蓝型油菜文库 (EMBL3) 中分离出来。克隆的基因 cruA 具有三个外显子并包括 5' 和 3' 侧翼区域。 cruA 编码区的核苷酸序列与用于筛选文库的 cDNA 相同 (1)。显示了 TATA 盒、转录起始位点、翻译起始点和聚腺苷酸化信号,以及 TATA 盒 5' 端的四个区域,这些区域与甘蓝型油菜的另一种胚胎特异性基因 napin 的启动子具有同源性 (2)。
A clone containing the complete gene of the embryo-specific storage protein cruciferin, has been isolated from a Brassica napus library (EMBL3). The cloned gene, cruA, has three exons and includes 5' and 3' flanking regions. The nucleotide sequence of the coding region of cruA is identical to the cDNA used to screen the library (1). A TATA box, transcription start site, translation start, and polyadenylation signals are indicated, as well as four regions 5' to the TATA box which have homology to the promoter of napin, another embryo-specific gene of Brassica napus (2).