COORDINATE REGULATION OF VIRULENCE GENES IN LISTERIA-MONOCYTOGENES REQUIRES THE PRODUCT OF THE PRFA GENE
COORDINATE REGULATION OF VIRULENCE GENES IN LISTERIA-MONOCYTOGENES REQUIRES THE PRODUCT OF THE PRFA GENE
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DOI：
10.1128/jb.174.2.568-574.1992
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发表时间：
1992-01-01
影响因子：
3.2
通讯作者：
NOTERMANS, S
中科院分区：
文献类型：
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作者：
CHAKRABORTY, T;LEIMEISTERWACHTER, M;NOTERMANS, S
单核增生李斯特菌的prfA基因编码一种激活李斯特溶菌素基因（lisA）转录的蛋白质。为了探索prfA基因产物在李斯特菌感染发病机制中的作用，我们通过染色体整合含有部分prfA编码区的新型自杀载体，在prfA中构建了一个位点定向插入突变。这一突变不仅使李斯特菌溶解素（lisA）基因转录沉默，而且还消除了与磷脂酰肌醇特异性磷脂酶C （pic）和金属蛋白酶（mpl）基因对应的特异性RNA转录物的产生，这两个基因是致病李斯特菌菌株仅表达的毒力基因产物。当在小鼠李斯特菌感染模型中进行测试时，也发现该菌株是无毒的。在小鼠感染模型中，多种特征（如LisA、Pic、Mpl的产生）的丧失和毒力的丧失是单个基因突变的结果，这表明prfA基因产物是单核增生乳杆菌多种毒力决定因素的积极调节因子。
The prfA gene of Listeria monocytogenes encodes a protein that activates transcription of the listeriolysin gene (lisA). In order to explore the role of the prfA gene product in the pathogenesis of listerial infection, we constructed a site-directed insertion mutation in prfA by the chromosomal integration of a novel suicide vector containing a portion of the prfA coding region. This mutation not only transcriptionally silenced the listeriolysin (lisA) gene but also abrogated production of specific RNA transcripts corresponding to the phosphatidylinositol-specific phospholipase C (pic) and metalloprotease (mpl) genes, two further virulence gene products expressed only by pathogenic Listeria strains. The strain was also found to be avirulent when tested in a mouse model of listerial infection. The concomitant loss of multiple characteristics such as production of LisA, Pic, Mpl, and loss of virulence in a mouse infection model is the result of a mutation in a single gene and demonstrates that the prfA gene product is a positive regulator of multiple virulence determinants in L. monocytogenes.