Highly efficient retrovirus-mediated gene transfer into rat hepatocytes in vivo
Highly efficient retrovirus-mediated gene transfer into rat hepatocytes in vivo
复制标题
DOI：
10.1089/hum.1997.8.12-1491
复制
发表时间：
1997-08-10
影响因子：
4.2
通讯作者：
Ferry, N
中科院分区：
文献类型：
--
作者：
Kitten, O;Cosset, FL;Ferry, N
我们使用高滴度（10(8)ffu/ml）重组逆转录病毒载体在体内将p -半乳糖苷酶（P-Gal）基因转移到大鼠肝细胞。在部分肝切除术后第二天在门静脉血流中注射两次的动物中，一半的肝细胞（46 +/- 17%）在肝再生结束时表达该标记物。在整个肝脏中，阳性细胞的数量与病毒滴度以及P-Gal酶活性密切相关。由于已知通过逆转录病毒载体在肝脏中转移的基因是永久表达的，因此我们目前的结果为利用重组逆转录病毒载体开发遗传性肝病的基因治疗方案开辟了新的可能性。
We have used high-titer (10(8) ffu/ml) recombinant retroviral vectors to transfer the P-galactosidase (P-Gal) gene to rat hepatocytes in vivo. In animals injected twice in the portal blood stream the next day after partial hepatectomy, half of the hepatocytes (46 +/- 17%) expressed the marker at the end of liver regeneration. The number of positive cells closely correlated with the viral titer as well as with P-Gal enzymatic activity present in the whole liver, Because genes transferred via retroviral vectors in the liver are known to be expressed permanently, our present results open new possibilities for the development of gene therapy protocols for hereditary liver diseases using recombinant retroviral vectors.