Population structure-guided profiling of antibiotic resistance patterns in clinical Listeria monocytogenes isolates from Germany identifies pbpB3 alleles associated with low levels of cephalosporin resistance
Population structure-guided profiling of antibiotic resistance patterns in clinical Listeria monocytogenes isolates from Germany identifies pbpB3 alleles associated with low levels of cephalosporin resistance
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DOI：
10.1080/22221751.2020.1799722
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发表时间：
2020-01-01
影响因子：
13.2
通讯作者：
Halbedel, Sven
中科院分区：
文献类型：
--
作者：
Fischer, Martin A.;Wamp, Sabrina;Halbedel, Sven
在过去的十年中，德国和欧盟的脊髓灰质炎疾病数量一直在增加。此外，关于单核细胞增生李斯特菌在临床和环境分离株中发生抗生素耐药性的报道越来越多。选择临床分离株进行14种抗生素敏感性试验。单核细胞生成分离物，以获得更精确的发展和表现的抗生素耐药性的L。单核细胞生成群根据核心基因组多位点序列分型（cgMLST）确定的种群结构，从2009年至2019年在德国收集的1220株测序菌株中选择了544株，以涵盖临床上观察到的系统发育多样性。单核细胞生成群所有检测的分离株均对氨苄青霉素、青霉素和复方新诺明敏感，这是治疗淋病最相关的抗生素。在544株分离株中，分别有493株（91%）和71株（13%）对达托霉素和环丙沙星耐药。虽然所有测试的菌株都显示出对头孢曲松的抗性，但它们的抗性水平在4 mg/L和>128 mg/L之间变化很大。青霉素结合蛋白基因epbpB 3的等位基因变异被确定为头孢曲松耐药水平差异的原因。这项研究是第一个群体结构指导的分析，在最近的临床分离的抗菌药物耐药性，并证实了青霉素结合蛋白B3（PBP B3）的重要性，为高水平的内在头孢菌素耐药的L。单核细胞生成在人群范围内。
Numbers of listeriosis illnesses have been increasing in Germany and the European Union during the last decade. In addition, reports on the occurrence of antibiotic resistance inListeria monocytogenesin clinical and environmental isolates are accumulating. The susceptibility towards 14 antibiotics was tested in a selection of clinicalL. monocytogenesisolates to get a more precise picture of the development and manifestation of antibiotic resistance in theL. monocytogenespopulation. Based on the population structure determined by core genome multi locus sequence typing (cgMLST) 544 out of 1220 sequenced strains collected in Germany between 2009 and 2019 were selected to cover the phylogenetic diversity observed in the clinicalL. monocytogenespopulation. All isolates tested were susceptible towards ampicillin, penicillin and co-trimoxazole - the most relevant antibiotics in the treatment of listeriosis. Resistance to daptomycin and ciprofloxacin was observed in 493 (91%) and in 71 (13%) of 544 isolates, respectively. While all tested strains showed resistance towards ceftriaxone, their resistance levels varied widely between 4 mg/L and >128 mg/L. An allelic variation of the penicillin binding protein genepbpB3was identified as the cause of this difference in ceftriaxone resistance levels. This study is the first population structure-guided analysis of antimicrobial resistance in recent clinical isolates and confirms the importance of penicillin binding protein B3 (PBP B3) for the high level of intrinsic cephalosporin resistance ofL. monocytogeneson a population-wide scale.