Reactions of macrophages exposed to particles < 10 μm
Reactions of macrophages exposed to particles < 10 μm
复制标题
DOI：
10.1016/s0013-9351(02)00021-x
复制
发表时间：
2003-01-01
影响因子：
8.3
通讯作者：
Koller, T
中科院分区：
文献类型：
--
作者：
Monn, C;Naef, R;Koller, T
本研究描述了大鼠肺巨噬细胞暴露于聚四氟乙烯过滤器收集的环境空气颗粒< 10 mum （PM10）的水提取物中的细胞毒性作用和氧化自由基的产生以及促炎细胞因子肿瘤生长因子α (TNFalpha)的实验。这些颗粒是在瑞士的两个城市地点、一个农村地点和一个高山地点的四个季节中收集的。细胞毒性作用，确定为代谢活动的减少，发现颗粒提取物从所有地点和季节。综合所有地点和季节的数据，过滤器上的颗粒质量与毒性之间存在剂量-响应函数（r(2) = 0.633，线性回归）。促炎细胞因子TNFalpha和氧化自由基的释放在春夏秋季收集的颗粒中最为明显。而在蒙大拿（高山），细胞的刺激与过滤器上的粒子质量正相关，这种相关性在苏黎世和卢加诺的城市站点为负相关。据解释，在高PM10水平，如在这些城市，巨噬细胞被抑制增加空气污染由于毒性作用。用内毒素中和蛋白处理提取物可抑制细胞毒作用和氧化自由基的释放。这表明内毒素，革兰氏阴性菌的细胞壁成分，是调节巨噬细胞活性的因素之一。综上所述，实验表明PM10组分中存在水溶性巨噬细胞毒性和刺激巨噬细胞的化合物。这些数据至少为PM10的一些已知有害影响提供了解释，并证实内毒素可能是一种反应物。2002 Elsevier Science（美国）。版权所有。
This study describes experiments on cytotoxic effects and the production of oxidative radicals and the proinflammatory cytokine tumor growth factor alpha (TNFalpha) in a cell line of rat lung macrophages exposed to aqueous extracts from ambient air particles < 10 mum (PM10) collected on Teflon filters. The particles were collected during the four seasons at two urban sites, one rural site, and one alpine site in Switzerland. Cytotoxic effects, determined as a reduction in the metabolic activity, were found in particle extracts from all sites and seasons. Taking together the data from all sites and seasons, a dose-response function was observed between the particle mass on the filter and toxicity (r(2) = 0.633, linear regression). The release of the pro-inflammatory cytokine TNFalpha as well as of oxidative radicals was most pronounced in particles collected in spring-summer and autumn. While at Montana (alpine), the stimulation of the cells was positively correlated with the particle mass on the filters, this correlation was negative at the urban sites Zurich and Lugano. It is interpreted that at high PM10 levels, as in these cities, macrophages are inhibited by increasing air pollution due to toxic effects. Cytotoxic effects and the release of oxidative radicals could be inhibited when the extracts were treated with an endotoxin-neutralizing protein. This suggests that endotoxin, a cell-wall constituent of gram-negative bacteria, is one of the factors which modulates macrophage activity. All together, the experiments indicate that in the PM10 fraction, water-soluble macrophage-toxic and macrophage-stimulating compounds are present. The data offer an explanation for at least some of the known harmful effects of PM10 and confirm endotoxin as a possible reactant. 2002 Elsevier Science (USA). All rights reserved.