Relationship between midcycle luteinizing hormone surge quality and oocyte fertilization
Relationship between midcycle luteinizing hormone surge quality and oocyte fertilization
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DOI：
10.1016/s0015-0282(99)00481-1
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发表时间：
2000-01-01
影响因子：
6.7
通讯作者：
Hull, MGR
中科院分区：
文献类型：
--
作者：
Verpoest, WMJA;Cahill, DJ;Hull, MGR
目的：探讨排卵前卵泡液中黄体生成素、卵泡刺激素和类固醇激素水平的变化与受精的关系。设计：前瞻性研究结果的回顾分析。地点：某大学附属医院生殖医学门诊。患者(S)：不孕症患者，无刺激，体外受精研究周期明显规律。干预(S)：用荧光免疫法测定排卵前卵泡液中促黄体生成素、卵泡刺激素、雌二醇性激素和血清黄体生成素水平。主要观察指标：卵母细胞受精率。结果(S)：可移植胚胎84枚(正常受精率和卵裂率，67%)，41个卵母细胞(33%)未受精。对匹配的卵泡刺激素的分析表明，卵裂胚胎的卵泡刺激素浓度的中位数(14.6IU/L)显著高于未受精的卵母细胞(10.4IU/L)。在有卵裂胚胎的周期中，血清黄体生成素浓度同样较高。两组卵泡刺激素(FSH)、雌二醇(E-2)、孕酮(P)水平差异无统计学意义。结论：尽管卵泡刺激素和类固醇水平正常，但排卵前卵泡刺激素水平降低与卵母细胞体外受精障碍有关。(Fertil Steril(R)2000；73：75-7.(C)1999年由美国生殖医学会提供。)
Objective: To determine whether alterations in preovulatory follicular fluid (FF) levels of LH, FSH, and steroids are associated with the probability of fertilization.Design: Retrospective analysis of prospective study results.Setting: Reproductive medicine clinic of a university teaching hospital.Patient(s): Infertile women, with unstimulated, apparently regular cycles in an IVF research program.Intervention(s): Measurement of preovulatory FF levels of LH, FSH, E-2, and P and serum LH levels by fluoroimmunometry.Main Outcome Measure: Oocyte fertilization.Result(s): There were 84 transferable embryos (rate of normal fertilization and cleavage, 67%), and 41 oocytes (33%) failed to fertilize. Analysis of the matched FF showed that the median concentration of FF LH was significantly higher for cleaving embryos than for unfertilized oocytes (14.6 vs. 10.4 IU/L). Serum LH concentrations were similarly higher in cycles with cleaving embryos. There were no statistically significant differences in FF concentrations of FSH, E-2, or P in the two groups.Conclusion: Reduced preovulatory FF LH levels are associated with impaired fertilization of oocytes in vitro, despite normal FF FSH and steroid levels. (Fertil Steril(R) 2000;73:75-7. (C) 1999 by American Society for Reproductive Medicine.)