Effect of the oestrous cycle, pregnancy and uterine region on the responsiveness of the isolated mouse uterus to prostaglandin F2α and the thromboxane mimetic U46619
Effect of the oestrous cycle, pregnancy and uterine region on the responsiveness of the isolated mouse uterus to prostaglandin F2α and the thromboxane mimetic U46619
复制标题
DOI：
10.1677/joe.1.06466
复制
发表时间：
2006-03-01
影响因子：
4
通讯作者：
Woodward, DF
中科院分区：
文献类型：
--
作者：
Griffiths, AL;Marshall, KM;Woodward, DF
本实验室先前的研究表明，非妊娠小鼠的离体子宫具有前列腺素F和血栓素受体群体，与人类子宫肌层中发现的相似。本研究的目的是研究在发情周期的不同阶段和妊娠期间，小鼠子宫中生肌活性和对前列腺素F-2 α（PGF（2 α））和血栓素模拟物U46619的反应性的任何区域变化。从BKW小鼠的子宫样品取自沿着每个子宫角长度的不同解剖学节段，并设置为在37 ℃下用Krebs溶液（含有1 μ M吲哚美辛）以2 ml/min灌注，并用95%O-2/5%CO2充气。反应（曲线下面积）表示为低渗休克诱导的最终收缩的百分比。数据表示为平均值S.E.M.。使用Student配对t检验或双因素ANOVA和Bonferroni事后检验进行分析。区域变化的生肌活性观察到在所有组织研究，除了在劳动过程中采取的。这些组织表现出显着更大的生肌活性比在妊娠后期和发情周期的所有阶段的组织。妊娠动物的组织通常比非妊娠动物的组织对U46619和PGF（2 α）更敏感。在发情周期中，子宫角上段的组织对两种激动剂的反应更强。研究结果表明，激素环境和切除部位是重要的生肌活性和反应。
Previous studies in this laboratory have suggested that the isolated uterus from non-pregnant mice has a prostaglandin F and a thromboxane receptor population similar to that found in human myometrium. The aim of this study was to investigate any regional variation in myogenic activity and responsiveness to prostaglandin F-2 alpha (PGF(2 alpha)) and tile thromboxane mimetic U46619 in the mouse uterus taken during different stages of the oestrous cycle and during pregnancy. Uterine samples from BKW mice were taken From different anatomical segments along the length of each uterine horn and set up for superfusion at 2 ml/min with Krebs solution (containing 1 mu M indometacin) it 37 degrees C, and gassed with 95%O-2/5%CO2. Responses (area under the Curve) are expressed as a percentage of the final contraction induced by hypotonic shock. Data are expressed as the means S.E.M. of n= 5-12 and were analysed using Student's paired t-test or two-way ANOVA with a Bonferroni post hoc test. Regional variation in myogenic activity was observed in all tissues studied except those taken during labour. These tissues displayed significantly greater myogenic activity than tissues taken at late gestation and at all stages of the oestrous cycle. Tissues from pregnant animals were generally more responsive to U46619 and PGF(2 alpha) than tissues taken from non-pregnant animals. Tissues taken from the upper segment of the uterine horn were more responsive to both agonists during the oestrous cycle. The findings demonstrated that the hormonal milieu and site of excision are important for myogenic activity and responsiveness.