Ex vivo cultured neuronal networks emit in vivo-like spontaneous activity
Ex vivo cultured neuronal networks emit in vivo-like spontaneous activity
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DOI：
10.1007/s12576-014-0337-4
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发表时间：
2014-11-01
影响因子：
2.3
通讯作者：
Ikegaya, Yuji
中科院分区：
文献类型：
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作者：
Okamoto, Kazuki;Ishikawa, Tomoe;Ikegaya, Yuji
自发的神经元活动几乎存在于所有的大脑区域，但其功能和时空模式都没有得到充分的了解。体外器官型切片培养可能为研究自发活动的某些方面提供了机会，因为它们可以自我恢复在切片过程中崩溃的网络。在海马体网络中，我们比较了体内自发活动与急性和培养切片的水平和模式。我们发现体内海马的放电速率和兴奋性突触活动与切片培养相比更相似。软置信度加权算法是一种没有人为偏见的机器学习技术，它还揭示了海马切片培养在自发活动参数的总体趋势方面与体内海马相似。
Spontaneous neuronal activity is present in virtually all brain regions, but neither its function nor spatiotemporal patterns are fully understood. Ex vivo organotypic slice cultures may offer an opportunity to investigate some aspects of spontaneous activity, because they self-restore their networks that collapsed during slicing procedures. In hippocampal networks, we compared the levels and patterns of in vivo spontaneous activity to those in acute and cultured slices. We found that the firing rates and excitatory synaptic activity in the in vivo hippocampus are more similar to those in slice cultures compared to acute slices. The soft confidence-weighted algorithm, a machine learning technique without human bias, also revealed that hippocampal slice cultures resemble the in vivo hippocampus in terms of the overall tendency of the parameters of spontaneous activity.