The Reconfigurable Maze Provides Flexible, Scalable, Reproducible, and Repeatable Tests
The Reconfigurable Maze Provides Flexible, Scalable, Reproducible, and Repeatable Tests
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DOI：
10.1016/j.isci.2019.100787
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发表时间：
2020-01-24
期刊：
影响因子：
5.8
通讯作者：
Takahashi, Susumu
中科院分区：
文献类型：
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作者：
Hoshino, Satoshi;Takahashi, Riku;Takahashi, Susumu
多个迷宫经常被用来测试动物的表现，因为每个迷宫都有其形状固有的缺点。然而，迷宫形状不能在单一环境中以可重复和可扩展的方式灵活快速地复制。在这里，为了克服缺乏灵活性、可伸缩性、重复性和可重复性，我们开发了一个可重新配置的迷宫系统，该系统由联锁跑道和一系列附属部件组成。它允许实验者快速灵活地沿网格图案以可重复和可扩展的方式配置各种迷宫结构。空间导航行为和海马区位置编码不受联锁机制的影响。作为一个原理证明，我们证明了迷宫变形导致了空间感受野的位置重新映射。因此，可重新配置的迷宫提供了灵活性、可伸缩性、重复性和再现性，从而促进了对学习和记忆的神经元底物的一致研究，并允许筛选行为表型。
Multiple mazes are routinely used to test the performance of animals because each has disadvantages inherent to its shape. However, the maze shape cannot be flexibly and rapidly reproduced in a repeatable and scalable way in a single environment. Here, to overcome the lack of flexibility, scalability, reproducibility, and repeatability, we develop a reconfigurable maze system that consists of interlocking runways and an array of accompanying parts. It allows experimenters to rapidly and flexibly configure a variety of maze structures along the grid pattern in a repeatable and scalable manner. Spatial navigational behavior and hippocampal place coding were not impaired by the interlocking mechanism. As a proof-of-principle demonstration, we demonstrate that the maze morphing induces location remapping of the spatial receptive field. The reconfigurable maze thus provides flexibility, scalability, repeatability, and reproducibility, therefore facilitating consistent investigation into the neuronal substrates for learning and memory and allowing screening for behavioral phenotypes.