Bioreactor studies on the endophytic fungus Entrophospora infrequens for the production of an anticancer alkaloid camptothecin
Bioreactor studies on the endophytic fungus Entrophospora infrequens for the production of an anticancer alkaloid camptothecin
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DOI：
10.1139/w05-122
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发表时间：
2006-03-01
影响因子：
2.8
通讯作者：
Qazi, GN
中科院分区：
文献类型：
--
作者：
Amna, T;Puri, SC;Qazi, GN
来自生长在印度查谟和马哈巴莱什瓦尔地区的Nothapodytes foetida的枝条（年轻和年老）用于分离52株内生真菌，并测试它们产生抗癌生物碱喜树碱的能力。其中一个分离物是从白刺内树皮组织中分离的。发现生长在印度J&K州查谟地区的foetida植物在半合成液体培养基中生长时产生可检测量的喜树碱及其衍生物。通过理化分析鉴定了喜树碱，并通过光谱研究进一步证实。在零时间培养物或未接种的培养肉汤中未检测到喜树碱。在摇瓶中96小时内喜树碱的最大产量为0.575 +/- 0.031 mg/100 g干细胞质量，而在生物反应器中48小时内记录到4.96 +/- 0.73 mg/100 g干细胞质量。
Twigs (young and old) from Nothapodytes foetida growing in the Jammu and Mahabaleshwar regions in India were used for the isolation of 52 strains of endophytic fungi and were tested for their ability to produce the anticancer alkaloid camptothecin. One of the isolates from the inner bark tissue of the N. foetida plant growing in the Jammu region of J&K state, India, was found to produce detectable quantities of camptothecin and its derivatives when grown in a semi-synthetic liquid medium. Camptothecin was identified by physicochemical analysis and further confirmed by spectroscopic studies. No camptothecin was detected in zero time cultures or in uninoculated Culture broth. The maximum yield of camptothecin was 0.575 +/- 0.031 mg/100 g of dry cell mass in 96 h in shake flasks, whereas 4.96 +/- 0.73 mg/100 g of dry mass was recorded in 48 h in a bioreactor.