Receptors for the neuropeptides, myoinhibitory peptide and SIFamide, in control of the salivary glands of the blacklegged tick Ixodes scapularis
Receptors for the neuropeptides, myoinhibitory peptide and SIFamide, in control of the salivary glands of the blacklegged tick Ixodes scapularis
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DOI：
10.1016/j.ibmb.2013.01.002
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发表时间：
2013-04-01
影响因子：
3.8
通讯作者：
Park, Yoonseong
中科院分区：
文献类型：
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作者：
Simo, Ladislav;Koci, Juraj;Park, Yoonseong
蜱的唾液腺是蜱吸血的重要器官。我们之前描述了一对产生肌抑制肽（MIP）和SIFamide的原脑唾液腺神经元对II型和III型唾液腺泡的神经支配（Simo等人，2009年b）。在这项研究中，我们确定了这些神经肽在唾液腺中表达的真实受体。这些受体的肩胛硬蜱基因组序列中的同源性为基础的搜索，其次是基因克隆和功能表达的受体。两种受体都被纳摩尔浓度的各自配体激活。两种配体及其各自受体的时间表达模式表明，SIFamide信号系统预先存在于未进食的唾液腺中，而MIP系统在进食开始时被激活。在II型和III型腺泡中检测到唾液腺中SIFamide受体的免疫反应性，其围绕腺泡瓣膜并延伸至腺泡腔的基底区域。SIFamide受体在唾液腺中的位置提示了三种潜在的靶细胞类型及其可能的功能：可能在腺泡收缩和/或瓣膜控制中起作用的肌上皮细胞;用于调节旁分泌多巴胺的大的基底多巴胺能颗粒细胞;以及可能参与腺泡导管及其瓣膜控制的颈细胞。(C)2013爱思唯尔有限公司保留所有权利。
Tick salivary glands are important organs that enable the hematophagous feeding of the tick. We previously described the innervation of the salivary gland acini types II and III by a pair of protocerebral salivary gland neurons that produce both myoinhibitory peptide (MIP) and SIFamide (Simo et al., 2009b). In this study we identified authentic receptors expressed in the salivary glands for these neuropeptides. Homology-based searches for these receptors in the Ixodes scapularis genome sequence were followed by gene cloning and functional expression of the receptors. Both receptors were activated bylaw nanomolar concentrations of their respective ligands. The temporal expression patterns of the two ligands and their respective receptors suggest that the SIFamide signaling system pre-exists in unfed salivary glands, while the MIP system is activated upon initiation of feeding. Immunoreactivity for the SIFamide receptor in the salivary gland was detected in acini types II and III, surrounding the acinar valve and extending to the basal region of the acinar lumen. The location of the SIFamide receptor in the salivary glands suggests three potential target cell types and their probable functions: myoepithelial cell that may function in the contraction of the acini and/or the control of the valve; large, basally located dopaminergic granular cells for regulation of paracrine dopamine; and neck cells that may be involved in the control of the acinar duct and its valve. (C) 2013 Elsevier Ltd. All rights reserved.