MOLECULAR CHARACTERIZATION AND EXPRESSION OF THE (NA+ + K+)-ATPASE ALPHA-SUBUNIT IN DROSOPHILA-MELANOGASTER
MOLECULAR CHARACTERIZATION AND EXPRESSION OF THE (NA+ + K+)-ATPASE ALPHA-SUBUNIT IN DROSOPHILA-MELANOGASTER
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DOI：
10.1002/j.1460-2075.1989.tb03364.x
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发表时间：
1989-01-01
期刊：
影响因子：
11.4
通讯作者：
FAMBROUGH, DM
中科院分区：
文献类型：
--
作者：
LEBOVITZ, RM;TAKEYASU, K;FAMBROUGH, DM
（Na+ + K+）-ATP酶（钠泵）是一种对哇巴因敏感的产电离子泵，负责维持几乎所有动物细胞中钠和钾离子的平衡。在组织培养的果蝇细胞中发现了对哇巴因敏感的铷摄取，表明钠泵，当喂食含有哇巴因的饮食时，幼虫和成虫都死亡。禽钠泵α-的单克隆抗体发现亚基与果蝇钠泵α-发生交叉反应亚单位 免疫荧光显微镜被用来获得钠泵在果蝇组织中的表达的半定量视图：在malphigian小管，间接飞行肌和管状肌，以及整个神经系统中检测到高水平的钠泵。编码该钠泵α-的cDNA亚基的基因克隆、测序并在小鼠L细胞中表达。在氨基酸水平上，其推导的1038个残基的序列（无脊椎动物的第一个这样的序列）与α-亚基序列报告的脊椎动物。果蝇中只发现了一个基因，位于第三条染色体的93 B位置。已经发现限制性位点多态性，并且存在几种可能涉及α-谷氨酰胺酶的突变。亚基基因
The (Na+ + K+)-ATPase (sodium pump) is an ouabain-sensitive, electrogenic ion pump responsible for maintaining the balance of sodium and potassium ions in almost all animal cells. Robust, ouabin-sensitive rubidium uptake, indicative of the sodium pump, was found in tissue-cultured Drosophila cells, and both larvae and adults die when fed a diet containing ouabain. A monoclonal antibody to the avian sodium pump .alpha.-subunit was found to cross-react with the Drosophila sodium pump .alpha.-subunit. Immunofluorescence microscopy was used to obtain a semi-quantitative view of the expression of the sodium pump in Drosophila tissues: high levels of the sodium pump were detected in malphigian tubules, indirect flight muscles and tubular muscles, and throughout the nervous system. The cDNA encoding this sodium pump .alpha.-subunit in Drosophila melanogaster was cloned, sequenced and expressed in mouse L cells. At the amino acid level, its deduced sequence of 1038 residues (the first such sequence for an invertebrate) is .apprx.80% similar to .alpha.-subunit sequences reported for vertebrates. Only one gene was found in Drosophila, located on the third chromosome at position 93B. A restriction site polymorphism has been found, and several mutations exist that may involve the .alpha.-subunit gene.