Enzyme encapsulation in microparticles composed of polymerized ionic liquids for highly active and reusable biocatalysts.
Enzyme encapsulation in microparticles composed of polymerized ionic liquids for highly active and reusable biocatalysts.
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DOI：
10.1039/b823064a
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发表时间：
2009-05
影响因子：
3.2
通讯作者：
K. Nakashima;N. Kamiya;D. Koda;T. Maruyama;M. Goto
中科院分区：
文献类型：
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作者：
K. Nakashima;N. Kamiya;D. Koda;T. Maruyama;M. Goto
辣根过氧化物酶（HRP）被包裹在聚合离子液体微粒（pIL-MP）中，该微粒是由1-乙烯基-3-乙基咪唑溴在交联剂N，N'-亚甲基双（丙烯酰胺）存在下在浓油包水（W/O）乳液中聚合而成的。用梳状聚乙二醇（PM(13)-HRP）修饰的pIL-MP包封HRP在水溶液中表现出优异的愈创木酚氧化活性。在pIL-MP中，PM(13)-HRP的活性比包裹在聚丙烯酰胺微粒中的酶高2倍以上。随着交联剂浓度的增加，催化活性下降，这可能是由于抑制了底物的扩散。在pIL-MP中，PM(13)-HRP的活性取决于凝胶的外部环境（即pH和温度）。通过离心可以很容易地从反应混合物中回收pIL-MP，这使得生物催化剂可以循环用于重复氧化反应。
Horseradish peroxidase (HRP) is encapsulated in polymerized ionic liquid microparticles (pIL-MP), which are prepared by polymerization of 1-vinyl-3-ethylimidazolium bromide in the presence of the crosslinker N,N'-methylenebis(acrylamide) in a concentrated water-in-oil (W/O) emulsion. pIL-MP encapsulating HRP chemically-modified with comb-shaped polyethylene glycol (PM(13)-HRP) exhibit excellent activity for guaiacol oxidation in an aqueous solution. The PM(13)-HRP in pIL-MP shows more than 2-fold higher activity than that of the enzyme encapsulated in a polyacrylamide microparticle. The catalytic activity declines with an increase in the crosslinker concentration of the pIL-MP, probably due to suppression of substrate diffusion. The activity of PM(13)-HRP in pIL-MP depends on the external environment of the gel (i.e. pH and temperature). The pIL-MP are easily recovered from the reaction mixture by centrifugation, which makes it possible to recycle the biocatalyst for repeated oxidation reactions.